Chinese Journal Of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (3): 314-317.

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The effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation

YAN Guang-bin, Yu Nan-sheng, LU Wei-jie,LU Yong-hui, QIAN Dong-yang   

  1. Department of Orthopaedics,the First Affiliated Hospital of Guangzhou Medical College,Guangzhou 510120,China
  • Received:2013-03-10 Online:2013-05-25 Published:2013-06-06

Abstract:

Objective To investigate the effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation. Methods LIM mineralization protein overexpression lentiviral vector was constructed and transfected into osteosarcoma cell line OS-9901 cells by  liposome. Quantitative PCR and Western blot were performed to measure the relative mRNA and protein expression of LIM mineralization protein 1, and BrdU assay was performed to detect cell proliferation before and after the LMP-1 over-expression. Results LMP-1 mRNA and protein expression did not change significantly (P>0.05) at 6 h after cells transfected with the LMP-1 expression lentiviral vector. When LMP-1 mRNA and protein expression were significantly increased at 12h, 24h and 48h after cells transfected with the LMP-1 expression lentiviral vector, DNA synthesis was significantly lower (P<0.05). Conclusion LIM mineralization protein 1 overexpression can inhibit osteosarcoma cell line OS-9901 cell proliferation.

Key words: LIM mineralization protein 1, Osteosarcoma cells, Lentiviral vector

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