维生素C对MPP+致帕金森病细胞模型中PC12细胞凋亡的影响

Abstract

Abstract:

Objective　To investigate the effect and the related mechanism of Vitamin C (Vit C) on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's disease (PD).　Methods　PC12 cells were divided to MPP+ induced cell model of PD group (MPP+ group) and Vit C treatment group (Vit C group). Firstly, Vit C group was incubated with 250 μmol/L Vit C 6 h, and then the two groups were treated with 100 μmol/L MPP+ 12 h. The LDH concentration was measured by LDH kit. The TUNEL method was performed to measure PC12 cells apoptosis in two groups. The expression of Caspase3 and c-Caspase3 was detected by Western-blot. Results Compared with MPP+ group, Vit C significantly inhibited LDH concentration and decreased apoptosis of PC12 cells, indicating that Vit C could down-regulate the expression of c-Caspase3 (P<0.05).　Conclusion　Vit C can decrease apoptosis of PC12 cells in vitro significantlyand play important protection role in PD via down-regulation of the expression of c-Caspase3

Key words: Parkinson's disease, TUNEL method, Apoptosis, Vitamin C, MPP+

CLC Number:

R651.15
R962

CAO Jian, WANG Rui, WANG Qian, WANG Shuang. Effects of vitamin C on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's Disease[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(4): 453-456.

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Effects of vitamin C on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's Disease

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