胸苷激酶1（TK1）在骨肉瘤中的表达及其临床病理意义

Abstract

Abstract:

Objective To investigate the expression ofTK1 in osteosarcoma tumor cells and the TK1 level of TK1 in the serum of patients with osteosarcoma, and analyze their clinico-pathological significances. Methods　40 cases of osteosarcoma and 20 cases of giant cell tumor were included in this study. Immunohistochemical staining of TK1 was performed on the tumor tissue by MaxVision method. Serum TK1 level in 20 patients with osteosarcoma and 20 patients with bone trauma was tested by chemiluminescence dot blot enzyme chemistry. Results The reactivity assessed was in cytoplasm and nuclei. The positive rate of TK1 in high grade osteosarcoma was significantly higher than that in low grade osteasarcoma(P<0.05); The positive rate of TK1 in osteasarcomais was higher than that in giant cell tumor, and the difference was statistically significant(P<0.05). In addition, the serum TK1 content in patients with osteosarcoma is significantly higher than that in patients with bone traumatic [2.95 (0.97-7.25) pmol/Lvs 0.89 (0.32-2.58) pmol/L,P<0.05]. Conclusion TK1 has a certain value in the differential diagnosis of osteosarcoma from benign bone lesions. High level of TK1 can be tested in the serum in patient with osteosarcoma, and it seems that TK1 is a sensitive indicator for testing osteosarcoma proliferation.

Key words: Thymidine kinase 1, Osteosarcoma, Immunohistochemical, Chemiluminescence

CLC Number:

R738.1

HU Mei-Quan, WANG Da-Beng, XIONG Jian-Xi, GUAN Hong, TUN Dun-Hui, HUANG Yu-Hua, SHU Wei-Min, LI Wei, YANG Ya-Ji, ZHANG Ji-Jun. Expression of Thymidine kinase 1 (TK1) in osteosarcoma and its clinico-pathological significance[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(5): 564-567.

References

[1] Gesing A, Modrzejewska H, Karbownik M, et al. Thymidine kinase and adenosine kinase activities in homogenates of thyroid lobes in enmity roidectomized rats, effects of melatonin in vitro
[J]. Neuro Endocrinol Lett, 2000, 21 (6) : 453-459.

[2] Struthers H, Viertl D, Kosinski M, et al. Charge dependent substrate activity of C3' and N3 functionalized, organometallic technetium and rhenium-labeled thymidine derivatives toward human thymidine kinase 1
[J]. Bioconjug Chem, 2010, 21(4):622-634.

[3] Topolcan O, Holubec L Jr．The role of thymidine kinase in cancer diseases
[J]．Expert Opin Med Diagn, 2008, 2(2):129-141.

[4] Chen Z, Zhou H, Li S, et al．Serological thymidine kinase1(STK1) indicates an elevated risk for the development of malignant tumours
[J]．Anticancer Res, 2008, 28(6B):3897-3907.

[5] Green FL, Page DL, Fleming ID, Fritz AG, Balch CM, Haller DG. Morrow. AJCC Cancer Staging Manual
[M]. 6th ed. Springer. New York. 2002:133-137.

[6] Sobin LH, Witteking C. UICCTNM Classification of Malignant Tumours
[M].6th ed.Wiley: New York. 2002:735-737.

[7] Bradshaw Jr HD, Deininger PL. Human thymidine kinase gene:molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells
[J]. Mol Cell Biol, 1984,4(11):2316-2320.

[8] Welin M, Kosinska U, Mikkelsen N, et al. Structures of thymidine kinase 1 of human and mycoplasmic origin
[J]. Proc Natl Acad Sci USA, 2004,101(52):17970-17975.

[9] Mao Y, Wu J, Wang N, et al. A comparative study: immunohistochemical detection of cytosolic thymidine kinase and proliferating cell nuclear antigen in breast cancer
[J]. Cancer Invest, 2002, 20(7-8):922-931.

[10] 李艳,张平安,邹力,等.免疫印迹法测定乳腺肿瘤患者胸苷激酶
[J].中华检验医学杂志,2000, 23(6):334-336.

[11]Khan Z, Knecht W, Willer M, et al．Plant thymidine kinase 1 a novel efficient suicide gene for malignant glioma therapy
[J]．Neuro Oncol, 2010,12(6):549-558.

Expression of Thymidine kinase 1 (TK1) in osteosarcoma and its clinico-pathological significance

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 0

[1]	DONG Li-ping, WU Chang-chu, LI Jian-ming, LIU Guan-lan, YUAN Heng, WU Gan-qiu. The expression of nestin in human fetal digestive tract [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(4): 419-422.
[2]	. Expression of TAp73 and Beclin1 in human osteosarcoma and their clinical significance [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(1): 68-72.
[3]	YANG Lian-Jun, RONG Zi-Jie, ZHANG Zan-Jie, WU Guo-Feng, ZHANG Xi-Bing, WANG Jian, SHU Li-Xin. Synthesis and characterization of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres and observation of its inhibition on MG63 cells [J]. Chinese Journal of Clinical Anatomy, 2014, 32(5): 585-589.
[4]	YAN An-Bin, TU Nan-Sheng, LEI Wei-Jie, LEI Yong-Hui, JIAN Dong-Yang. The effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation [J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 314-317.
[5]	XUE Jian, JIN An-Min, LV Hai, MIN Shao-Xiong. The influence of down-regulation of TAp73 expression by short hairpin RNA on the proliferation and invasion of osteosarcoma [J]. Chinese Journal Of Clinical Anatomy, 2013, 31(2): 170-173.