豚鼠膀胱中二聚体Cajal间质细胞的分布特点及意义

doi:10.13418/j.issn.1001-165x.2014.01.011

Abstract

Abstract:

Objective　To observe the distribution of dimer interstitial cells of Cajal (ICC) in the different organizations and parts in guinea pig bladder.　Methods　The guinea pig bladder sections were made and the distribution of dimer ICC within different organizations were observed using electron microscope; The cryostat tissue sections of guinea pig bladder were made and dimer ICC marked with c-Kit antibody in different bladder parts were observed with immunofluorescence.　Results　Dimmer ICC, under electron microscope, was mainly distributed in the submucosa of detrusor while monomer ICC was mainly distributed between detrusor bundles. Under high-powered fields of confocal microscopy, the number of dimer ICC in bladder tip was 3.47±0.53 , bladder body was 1.57±0.45 and bladder neck was 0.49±0.19. Dimer ICC density in the bladder tip was significantly higher than that in the bladder body and neck (P <0.01).　Conclusion　Dimer ICC, mainly located in the submucosa of bladder tip, might accept the tension stimulation from mucous and then trigger the contraction of bladder tip as pacemaker cells.

Key words: Bladder, Interstitial cells of Cajal, Pacemaker cells

CLC Number:

R329.2

WANG Jiang-Beng, WANG Qi-Zhang, DING Guo-Fu. Distribution and significance of dimer interstitial cells of Cajal in the guinea pig bladder[J]. Chinese Journal of Clinical Anatomy, 2014, 32(1): 45-47.

References

[1] Kubota Y, Biers SM, Kohri K, et al. Effects of imatinib mesylate (Glivec) as a c-kit tyrosine kinase inhibitor in the guinea-pig urinary bladder
[J]. Neurourol Urodyn, 2006, 25(3): 205-210.

[2] Biers SM，Reynard JM，Doore T，et al．The functional effects of a c-kit tyrosine inhibitor on guinea-pig and human detrusor
[J]．BJU Int，2006，97(3)：612-616．

[3] Megan FK, Betty E, Lang RJ. Identification of the cells underlying pacemaker activity in the guinea-pig upper urinary tract
[J]. J Physiol,1999,519(Pt 3): 867-884.

[4] Shafik A，El-Sibai O，Shafik AA，et al．Identification of interstitial cells of Cajal in human urinary bladder：concept of vesical pacemaker
[J]．Urology，2004，64(4)：809-813．

[5] Gillespie JI, Harvey IJ, Drake MJ. Agonist- and nerve-induced phasic activity in the isolated whole bladder of the guinea pig: evidence for two types of bladder activity
[J]．Exp Am J Physiol Renal Physiol, 2003,88(3)：343-357．

[6] 王勤章，李云飞，丁国富，等．豚鼠膀胱Cajal样间质细胞类型及其与钙离子振荡特性的关系
[J]．中华泌尿外科杂志，2010，31(8) ：614-617.

[7] 王江平，王勤章，丁国富，等．膀胱二聚体Cajal间质细胞的超微结构及其对ATP的电反应
[J]．神经解剖学杂志，2012, 28(6): 617-620.

[8] Ward SM，Sanders KM．Pacemaker activity in septal structures of canine colonic circular muscle
[J]．Am J Physiol，1990，259(2)：264-273．

[9] Liu WC，Thuneberg L，Huizinga JD．Selective lesioning of interstitial cells of Cajal by methylene blue and light leads to loss of slow wave
[J].Am J Physiol，1994，266(3)：485-496．

[10]Biers SM, Reynard JM, Doore T, et al. The functional effects of a c-kit tyrosine inhibitor on guinea-pig and human detrusor
[J]. BJU Int, 2005，97(3):612 - 616.

[11]Kubota Y, Kojima Y, Shibata Y, et al. Role of KIT-positive interstitial cells of Cajal in the urinary bladder and possible therapeutic target for overactive bladder
[J]. Adv Urol, 2011，doi:10.1155/2011/816342.

[12]韩涛, 王勤章, 司军强，等．豚鼠膀胱不同区域逼尿肌的兴奋性观察
[J]. 山东医药, 2011， 51（42）: 29-30.

Distribution and significance of dimer interstitial cells of Cajal in the guinea pig bladder

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