Nix和miR-520h对神经胶质瘤发生的影响及相互作用

doi:10.13418/j.issn.1001-165x.2014.05.019

Abstract

Abstract:

Objective　To evaluate the biological function of Nix and its relation to microRNA miR-520h in glioma.　Methods　46 glioma specimens were selected to detect the expression of Nix by Western blotting and the microRNA expression by traditional RT-PCR．The shRNA were used to knockdown Nix of U251 glioblastomacell line, and the protein level of Nix was detected by Western blotting. In the presense of miR-520h inhibitor．The protein level of Nix and NF-κB was detected to evaluate their interaction in the U25l cell.　Results　Both RT-PCR analysis and Western blotting showed that high expression of miR-520h was always accompanied with high expression of Nix proteins. Under hypoxic conditions, Western blotting showed that levels of both Nix and p-NF-κB/p65 in Nix-wt U251 glioblastoma cells were significantly higher than those in Nix-kn U251 glioblastoma cells. Furthermore，200 nmol/L of has-miR-520h inhibitor caused low expression of Nix and high expression of p-NF-κB/p65 under hypoxic conditions.　Conclusion　MiR-520h is related to the expression of Nix protein in the tumorigenesis of glioma. MiR-520h may be a strong tumor activator through promoting Nix biosynthesis .

Key words: Glioma, Nix, miR-520h, NF-κB

CLC Number:

R739.41

HU Xiao-Fang, WANG Yong-Kuai, QIU Xiao-Zhong, WANG Guo-Bao. Study of the effect of Nix and miR-520h on glioma[J]. Chinese Journal of Clinical Anatomy, 2014, 32(5): 590-594.

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Study of the effect of Nix and miR-520h on glioma

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