Bax、Bcl-2在培养新生鼠皮层神经元中的表达及L-NAME的干预作用

doi:10.13418/j.issn.1001-165x.2014.05.020

Abstract

Abstract:

Objective　To investigate the expression of Bcl-2 and Bax in cultured cortical neurons after NMDA injury and intervention effect of L-NAME.　Methods 　The primary cortical neurons were identified by polyclonal antibody against NSE. The neurons were randomly divided into 3 groups: control group, NMDA-injured group, L-NAME pretreatment group. ELISA was employed to explore the expression of NOS, and the expression of Bcl-2 and Bax was detected by RT-PCR and Western blot. 　Results 　Immunofluorescence test showed that the most cells were NSE positive staining cells. NOS in L-NAME group were higher than that in control group, but lower than that in NMDA group; the expression of Bcl-2 mRNA and protein in L-NAME group were lower than in control group, but higher than that in NMDA group. The expression of Bax was in contrast with Bcl-2.　Conclusion　L-NAME plays an important protective role in neuronal apoptosis induced by NMDA, the possible mechanism of which may be related with up-regulation of Bcl-2 expression and down-regulation of Bax expression.

Key words: NOS, Bax, Bcl-2

CLC Number:

R363

SONG Hai-Yan, GAO Zhi-Chao, DENG Xiao-Hui, LIAN Hui, LIN Ming-Xin, FU Sheng-Qi. The expression of Bax and Bcl-2 in cultured neonatal rat cortical neurons and the role of L-NAME intervention[J]. Chinese Journal of Clinical Anatomy, 2014, 32(5): 595-598.

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The expression of Bax and Bcl-2 in cultured neonatal rat cortical neurons and the role of L-NAME intervention

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