人参皂苷Rg1对缺氧复氧BMSCs增殖和凋亡的影响

doi:10.13418/j.issn.1001-165x.2015.06.009

Abstract

Abstract:

Objective　To investigate the effects of ginsenoside Rg1 on BMSCs proliferation and apoptosis after acute hypoxia-reoxygenation（H/R）and the possible mechanism.　Methods　BMSCs were divided into normal control group，model group，ginsenoside Rg1 1×10-7，1×10-6 and 1×10-5 mol/L groups, Nimodipine 2.5×10-7 mol/L group（positive control group）. In the 12 hours before building H/R model，every groups were respectively added normal saline (normal control group, model group), ginsenoside Rg1 (ginsenoside Rg1 treated groups), Nimodipine ( positive control group). Acute H/R BMSCs model was established. TUNEL was used to count apoptosis rate of cell and IF and Western blot technique was used to observe the expression of PCNA、bcl-2、bax qualitatively and quantitatively. Results Normal control group showed no significant apoptotic cells，model group showed obvious apoptotic cells，compared with model group, the number of apoptotic cells of every groups decreased, Rg1 (1×10-5 mol/L) group was the most obvious. Compared with normal control group, the expression of PCNA and bcl-2 of model group decreased,but the expression of bax significantly increased, and the ratio of bcl-2/bax decreased; Compared with model group，the expression of PCNA and bcl-2 of ginsenoside Rg1 treated groups increased in a different degree,but the expression of bax showed the opposite trend; the ratio of bcl-2/bax increased , and ginsenoside Rg1 (1×10-5 mol/L) group was the most obvious. Conclusion Ginsenoside Rg1 can protect BMSCs after acute anoxia-reoxygenation by down-regulating the expression of bax and up-regulating the expression of PCNA and bcl-2 and by inhibiting apoptosis and promoting proliferation.

Key words: Ginsenoside Rg1, BMSCs, Proliferation, Apoptosis

References

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Effects of ginsenoside Rg1 on BMSCs proliferation and apoptosis after hypoxia-reoxygenation

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