Chinese Journal Of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (2): 202-207.doi: 10.13418/j.issn.1001-165x.2016.02.017

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Establishment and Identification of SETD4gene knockout mice

HUANG Sui, HUANG Meng-yi, ZHONG Yu-yun, LEI Ye-ming, ZHAO Shu-qi, CAI Jun-wei, JIANG Yong, LIU Jing-hua   

  1. Key laboratory for Functional Proteomics of Guangdong Province,Department of Pathophysiology,Southern Medical University,Guangzhou 510515
  • Received:2015-12-25 Online:2016-03-25 Published:2016-04-14

Abstract:

Objective To study the function of SETD4, the SETD4 gene knockout homozygous mice has been established.  Methods SETD4flox/+ mice and EIIa-Cre mice were interbred,the offspring of which was genotyping SETD4+/-.EIIa-Cre were crossed with C57BL/6 mice to obtain the mice with the SETD4+/- genotype,SETD4+/-  heterozygous mice were inbred and then the SETD4-/-  homozygous mice were gained. PCR was used to identify the genotype of the offspring,the expression of SETD4 mRNA was detected by RT-PCR and qPCR,and morphological changes of liver and lung were observed by HE staining. Result PCR results showed genotypes of the offspring of SETD4 gene knockout mice was in accordance with SETD4-/-. Compared with the wild type mice,expression of SETD4 mRNA in SETD4 gene knockout homozygous mice was significantly decreased,and morphological characteristics of liver and lung in SETD4 gene knockout homozygous mice had no significant changes. Conclusion Wehave successfully generated SETD4 gene knockout homozygous mice which can be used for study ofSETD4 function.

Key words:  SETD4, gene knockout, Cre/loxp, KO-First technology