The effect of anti-inflammatory treatment on the progress of diethylnitrosamine induced primary liver cancer

LI Xiang-zhi; NIU Hong-ling; LIU Xiao-long; LIU Li-xin

doi:10.13418/j.issn.1001-165x.2017.01.008

Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (1) : 37-42. DOI: 10.13418/j.issn.1001-165x.2017.01.008

The effect of anti-inflammatory treatment on the progress of diethylnitrosamine induced primary liver cancer

LI Xiang-zhi¹，NIU Hong-ling¹，LIU Xiao-long²，LIU Li-xin²

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Abstract

Objective　To investigate the influence of anti-inflammatory treatment on the progress of DEN induced primary liver cancer. 　Methods　42 rats which were fed with Diethylnitrosamine (DEN) solution to induce the rat model of liver cancer, and randomly divided into 3 groups. The nodule number and the structural change of liver tissue in each group were observed. CD68, CD206 and CD34 positive expression in liver tissue were detected by immunohistochemical staining in each group. Relative expression of TNF-α, IL10, TGF-β, HGF and NFκB in the liver tissue was detected by western blot in each group. Results　 Compared with the control group and glucurolactone group，hydrocortisone group's nodule number was fewer，the appearance time of liver cancer was postponed, the positive expression of CD68, CD206 and CD34 was fewer, the protein relative expression of TNF-α, IL10, TGF -β, HGF and NF kappa B was fewer at the 8th,10th, and 12th week.　Conclusion　Inhibiting the chronic nonspecific inflammation which is mediated by mononuclear - macrophage in liver microenvironment can delay the progress of the primary liver cancer in rats at an early stage which is induced by DEN.

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Anti-inflammatory treatment / Primary liver cancer / Diethylnitrosamine (DEN)

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LI Xiang-zhi，NIU Hong-ling，LIU Xiao-long，LIU Li-xin. The effect of anti-inflammatory treatment on the progress of diethylnitrosamine induced primary liver cancer[J]. Chinese Journal of Clinical Anatomy. 2017, 35(1): 37-42 https://doi.org/10.13418/j.issn.1001-165x.2017.01.008

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