HSNGLPL短肽修饰的聚氨酯的体内反应性分析

doi:10.13418/j.issn.1001-165x.2017.03.008

Chinese Journal Of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (3): 271-275.doi: 10.13418/j.issn.1001-165x.2017.03.008

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The bio-reactivity analysis of HSNGLPL peptide grafted-polyurethane

XIAO Jiang-wei¹, SHI Dan-dan¹, ZHU Rong¹, GU Rui-cai¹, WU Gang², LIAO Hua¹

1. Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering，Southern Medical University, Guangzhou 510515 China; 2. School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China

Received:2016-11-21 Online:2017-05-25 Published:2017-06-23

Abstract

Abstract:

Objective　To study the biocompatibility of HSNGLPL peptide grafted- polyurethane in vivo and in vitro.　Methods　HSNGLPL peptide solution, HSNGLPL peptide grafted polyurethane with different concentration, and BDO- PU are injected or implanted into tibialis anterior muscle of B6 mice, or co-cultured with C2C12 cell in vitro. CCK8, H&E, immunofleorecence was used to explore the material-induced inflammatory and immuno-reactivity in vitro and in vivo.　Results　HSNGLPL peptide grafted-polyurethane is biocompatible in vivo. BDO-PU induced a conspicuous monocytes/macrophages infiltration and myofiber degeneration, the inflammatory invasion and myofiber necrosis are mainly detected in the site around the implants. In contrast, peptide grafted-PUs induced intramuscular inflammation is more complex and sustained more than 56d. The number of apoptotic macrophages in muscle containing peptide-PU significantly lower than that containing BDO-PU.　Conclusions　HSNGLPL peptide grafted PUs triggers the more severe inflammation response than that of BDO-PU, and eventually change biocompatibility of PU material in vitro and in vivo.

Key words: Polyurethanes, 　HSNGLPL peptide;　Inflammation; , Immunity; , Infiltration

XIAO Jiang-wei, SHI Dan-dan, ZHU Rong, GU Rui-cai, WU Gang, LIAO Hua. The bio-reactivity analysis of HSNGLPL peptide grafted-polyurethane[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(3): 271-275.

References

[1] Rahimi A, Mashak A. Review on rubbers in medicine: natural, silicone and polyurethane rubbers[J]. Plastics Ruber and Composites, 2013, 42(6): 223-230.

[2] Shu JY, Xu T. 7.08-peptide-polymer conjugates toward functional hybrid biomaterials[J]. Polymer Science A Comprehensive Reference, 2012: 141-158.
[3] Shah RN, Shah NA, Del Rosario Lim MM, et al. Supramolecular design of self-assembling nanofibers for cartilage regeneration[J]. Proc Natl Acad Sci U S A, 2010, 107(8): 3293-3298.
[4] Zhou M, Wang W, Liao Y, et al. In vitro biocompatibility evaluation of silk-fibroin/polyurethane membrane with cultivation of HUVECs[J]. Frontiers of Materials Science, 2014, 8(1): 63-71.
[5] Gu Y, Chen L, Yang H L, et al. Evaluation of an injectable silk fibroin enhanced calcium phosphate cement loaded with human recombinant bone morphogenetic protein-2 in ovine lumbar interbody fusion[J]. J Biomed Mater Res A, 2011, 97(2): 177-185.
[6] Arnold L, Henry A, Poron F, et al. Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis[J]. J Exp Med, 2007, 204(5): 1057-1069.
[7] Charge SB, Rudnicki MA. Cellular and molecular regulation of muscle regeneration[J]. Physiol Rev, 2004, 84(1): 209-238.
[8] Anderson JM. Chapter 4 Mechanisms of inflammation and infection with implanted devices [J]. Cardiovasc Pathol,1993, 2(3, Supplement): 33-41.
[9] Guelcher SA. Biodegradable polyurethanes: synthesis and applications in regenerative medicine[J]. Tissue Eng Part B Rev, 2008, 14(1): 3-17.
[10]Santerre JP, Woodhouse K, Laroche G, et al. Understanding the biodegradation of polyurethanes: from classical implants to tissue engineering materials[J]. Biomaterials, 2005, 26(35): 7457-7470.
[11]Choi W S, Bae J W, Lim H R, et al. RGD peptide-immobilized electrospun matrix of polyurethane for enhanced endothelial cell affinity[J]. Biomed Mater, 2008, 3(4): 44104.

The bio-reactivity analysis of HSNGLPL peptide grafted-polyurethane

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