樟芝酵素对苯丙胺致PC12细胞毒性损伤的保护作用

doi:10.13418/j.issn.1001-165x.2017.04.009

Abstract

Abstract:

Objective To investigate the protective effect and its mechanism of Antrodia camphorata enzyme on the cytotoxicity injury of PC12 cells induced by Amphetamine (AMPH). Methods PC12 cells were treated with 3.5 mmol/L AMPH or 3.5 mmol/L AMPH pus Antrodia camphorata enzyme. The morphological changes of the cells were observed under the light microscope. Cell viability assay was measured by MTT method. Caspase-3 levels were detected by Western blot, and the changes of reactive oxygen species（ROS） and apoptosis of cells were analyzed by flow cytometer. Results (1)AMPH damaged structure of PC12 cells, and resulted in cell apoptosis. Antrodia camphorata enzyme reduced cell apoptosis, and increased the survival rate of cells. (2)Antrodia camphorata enzyme reduced generation of ROS in PC12 cells. (3)Compared to AMPH group, procaspase-3 (35 kd) level was increased while procaspase-3 (17 kd) level was reduced in Antrodia camphorata enzyme group. Conclusion Antrodia camphorata enzyme has a protective effect on the cytotoxicity of PC12 cells induced by AMPH. Its mechanism may be related to inhibition of the mitochondrial apoptosis signal pathway and reduction of reactive oxygen species in cells.

Key words: Antrodia camphorata enzymes, Amphetamine; , PC12 cell; , Apoptosis; , Reactive oxygen species

CHI Dan-lu1， SUN Yu1， PAN San-qiang2. The protective effect and its mechanism of Antrodia camphorata enzyme on the cytotoxicity injury of PC12 cells induced by amphetamine[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(4): 397-401.

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The protective effect and its mechanism of Antrodia camphorata enzyme on the cytotoxicity injury of PC12 cells induced by amphetamine

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