小鼠前脑线粒体蛋白的增龄性变化

doi:10.13418/j.issn.1001-165x.2018.04.012

Abstract

Abstract:

Objective To investigate the changes of mitochondrial protein expression in different stages of mouse forebrain development. Methods The forebrain tissues of fetal mice with 12.5 and 17.5 days pregnancy (E12.5, E17.5), 2-day-old neonatal mice , 3-week-old young mice and 2-month-old mice were collected to detect the expression levels of COX IV, HSP60, OGDH, PDHE1α mRNA and protein in different development stages by QRT-PCR and Western blotting. Results The mRNA level of COX IV was low in forebrain tissue from E12.5 to 2 days, and it increased significantly and reached the peak at 3 weeks. The mRNA levels of HSP60 and PDHE1α were significantly increased at E17.5, and the OGDH mRNA level had an obvious rise at 2 days, and all three reached the peak at 2 months. The proteins of COX IV, HSP60 and OGDH showed no significant difference from E12.5 to 3 weeks, but increased evidently at 2 months; and the protein of PDHE1α increased significantly with forebrain development and peaked at 2 months. In addition, there was a positive correlation between mRNA and protein expression levels of COX IV, HSP60, OGDH, PDHE1α. Conclusion COX IV, HSP60, OGDH and PDHE1α may be involved in mouse forebrain development and be closely related to age.

Key words: Mouse, 　Forebrain, 　Development, 　Mitochondrial protein

LV Jing, DENG Li, GUAN Wei-kang, ZHU Yan, GUO Kan, GAO Xiao-qing, YANG Chao-xian. Expression changes of mitochondrial protein with age in mouse forebrain development[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(4): 414-418.

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Expression changes of mitochondrial protein with age in mouse forebrain development

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