虫草素联合谷氨酰胺对LPS诱导的脓毒症大鼠炎症失衡及肝肺病理变化的影响

doi:10.13418/j.issn.1001-165x.2019.06.007

Abstract

Abstract: Objective To study the effects on inflammatory imbalance and pathological changes of liver and lung of cordycepin (Cop) combined with glutamine (Gln) in sepsis rats induced by lipopolysaccharide (LPS) and its possible mechanism. Methods Rats were randomly divided into five groups according to body weight: a control group, a model group (LPS group), a LPS+Cop group, a LPS+Gln group and a LPS+Cop+Gln group. The sepsis rat model was established by intraperitoneal injection of LPS (5 mg/kg). The levels of inflammatory factors IL-6, TNF-α, IL-1β and IL-10 in peripheral blood were detected by ELISA. The pathological damage of liver and lung tissue were observed by HE staining. The apoptosis rate of liver and lung tissue were detected by TUNEL staining. The expression of Caspase-3 and the phosphorylation of NF-κB p65 in liver and lung tissue were detected by Western blot. Results In the LPS+Cop group, LPS+Gln group and LPS+Cop+Gln group, reversal of high expressions of LPS-induced pro-inflammatory factors (IL-6, TNF-α, IL-1β) and low expression of anti-inflammatory factors (IL-10) could be observed (P<0.05). Also, alleviation of pathological damage, inhibition of apoptosis (P<0.05), reduction of high expression of apoptotic protein Caspase-3 (P<0.05) and down-regulation the phosphorylation level of NF-κB p65 (P<0.05) were observed. Conclusions Cordycepin combined with glutamine can effectively improve inflammation imbalance and pathological changes of liver and lung in LPS-induced sepsis rats.

Key words: Cordycepin; , Glutamine;　Lipopolysaccharides;　Sepsis;　NF-κB p65

CLC Number:

R285.5

FAN Xiao-guang, PENG Jin, HONG Shu-kun, HU Guo-xin, LIU Jian, ZHANG Zhi-kun, ZHAO Li-shu, WANG Heng, YANG Guang-hu, QIAO Lu-jun. Effects on inflammatory imbalance and pathological changes of liver and lung of cordycepin combined with glutamine in LPS-induced sepsis rats[J]. Chinese Journal of Clinical Anatomy, 2019, 37(6): 638-643.

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Effects on inflammatory imbalance and pathological changes of liver and lung of cordycepin combined with glutamine in LPS-induced sepsis rats

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