Spastin促进海马神经元树突野的形成

doi:10.13418/j.issn.1001-165x.2020.02.015

Abstract

Abstract: Objective　To investigate the effect of microtubule-severing protein Spastin on the formation of dendritic field in hippocampal neurons.　Methods　Spastin was transfected into hippocampal neurons and the α-amino-3-hydroxyl-5-methyl-4-isoxazole propionic acid (AMPA) receptors on dendrites were detected by immunofluorescence. Whole-cell patch clamp was used to detect miniature excitatory postsynaptic currents (mEPSCs).　Results　Overexpression of Spastin promoted dendritic outgrowth and branch formation, while knockdown of Spastin inhibited dendritic outgrowth and formation of new branches. The results showed that Spastin significantly promoted the formation of and complexity of dendritic field compared with the control group (P<0.05). Sholl analysis results showed that Spastin increased the complexity of dendrites (P<0.05). Immunofluorescence results showed that overexpression of Spastin promoted the expression of AMPA receptor GluA2 subunit in dendrites, which was significantly different from the control group (P<0.05) , while knockdown of Spastin inhibited the expression of GluA2 in dendrites (P<0.05). Whole-cell patch clamp detection showed that overexpression of Spastin increased the amplitude and frequency of mEPSCs, however, knockdown of Spastin significantly decreased the amplitude and frequency of mEPSC (P<0.05).　Conclusions　Spastin promotes the formation of neuronal dendrites that are the functional dendrites.

Key words: Spastin, 　Microtubule, 　Dendritic field, 　AMPA receptor, 　Neuron

CLC Number:

R322.81

ZHANG Jia-qi, CHEN Li, LI Jiong, LI Su-mei, ZHANG Ji-feng, TENG Ji-jun, GUO Guo-qing. Spastin promotes the formation of dendritic field in hippocampal neurons[J]. Chinese Journal of Clinical Anatomy, 2020, 38(2): 176-182.

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Spastin promotes the formation of dendritic field in hippocampal neurons

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