长链非编码RNA PVT1通过靶向miR-190对宫颈癌HeLa细胞生存及转移的影响

doi:10.13418/j.issn.1001-165x.2020.04.010

Abstract

Abstract: Objective　To investigate the effects and mechanism of lncRNA PVT1 on the growth and migration of cervical cancer Hela cell via targeting miR-190.　Methods　The expression levels of lncRNA PVT1 and miR-190 in normal cervical cells and different cervical cancer cells were identified by qRT-PCR. The targeting relationship between lncRNA PVT1 and miR-190 was predicted by bioinformatics software. Cell proliferation was detected by MTT, cell growth was detected by clone formation assays, apoptosis was identified by Hoechst staining and flow cytometry, cell invasion was detected by Transwell, cell migration was detected by a scratch test; proliferation, apoptosis and epithelial-mesenchymal transition (EMT)-related molecular expression were verified by Western Blot. Cervical cancer HeLa cell was inoculated with subcutaneous xenografts of the nude mice after treated with sh-PVT1, and the related molecules expression of proliferation and apoptosis- in tumor tissues and tumor volume were detected.　Results　Compared with the normal cervical cells, lncRNA PVT1 was highly expressed in various cervical cancer cells. After interfering with PVT1, the expression level of miR-190 significantly up-regulated. LncRNA PVT1 and miR-190 had strong associativity, which was verified by bioinformatics and luciferase reporter system. Sh-PVT1 could effectively inhibit the proliferation, invasion and migration of cervical cancer cells and promote apoptosis (P<0.01). LncRNA PVT1 could effectively inhibit the related molecules expression of EMT- and reduce the transformation of cervical cancer cells into EMT. The above phenomenon was effectively reversed after the miR-190 inhibitor (P<0.01). In vivo experiments confirmed that PVT1 knockdown could effectively reduce tumor cell proliferation and promote cell apoptosis (P<0.01).　Conclusions　LncRNA PVT1 is highly expressed in cervical cancer. PVT1 knockdown can effectively relieve the inhibitory effect of PVT1 on miR-190, thereby reducing proliferation, invasion and migration of cervical cancer cells and promoting apoptosis.

Key words: LncRNA PVT1, 　miR-190, 　Cervical cancer, 　EMT, 　Growth, 　Apoptosis

CLC Number:

R737.33

DENG Yong-hong, ZHANG Li, WANG Jing, YAN Ai-hua . Effects of LncRNA PVT1 on the growth and migration of cervical cancer HeLa cells via targeting miR-190[J]. Chinese Journal of Clinical Anatomy, 2020, 38(4): 414-420.

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Effects of LncRNA PVT1 on the growth and migration of cervical cancer HeLa cells via targeting miR-190

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