基于柠檬酸合成的新型可降解螺钉影像学和组织学的研究

doi:10.13418/j.issn.1001-165x.2021.01.012

Abstract

Abstract: Objective　To evaluate the applicability and mechanism of citric acid copolymer - hydroxyapatite (POC-click-HA) screws in treatment of lateral femoral condyle fracture of canine.　Methods　Nine adult male Beagles (weighing 9~12 kg) were selected to prepare the models (AO classification type B1) of bilateral lateral femoral condyle fracture, the right side was fixed with POC-click-HA absorbable cannulated screws as an experimental group and left side with Poly-DL-Lactic acid(PDLLA) screws as a control group. At 4 weeks, 8 weeks, and 12 weeks after operation，Micro-CT and histological analysis were taken to observe the fracture healing. The osteogenesis effect of MC3T3 E1 cell were observed by the Kreb’s cycle inhibitors of the CPI-613 and exogenous citric acid (CA).　Results Micro-CT result showed that the combination of POC-click-HA screw and bone was weaker than PDLLA screw at 4 weeks after surgery. At 8 weeks, the two groups of screws were gradually integrated with the surrounding bone tissue, with no significant difference. At 12 weeks, the screw-bone tissues of both groups were well bonded. Histological staining analysis result showed that new bone was gradually formed around the screws in experimental group. The screws were degrading and new bone was growing in. However, there were still more collagen fibers around the screws in the control group, only a few new bones were seen, and the screws were not degraded. The results of cell culture showed that the citric acid in the medium significantly reduced after the addition of cpi-613 inhibitor. The higher concentration of inhibitor, the fewer ALP positive cells were formed. The higher the citric acid concentration, the more calcium nodules formed. Conclusions　Compared with PLLA screw, POC -click-HA bone screw has stronger bone induction ability and faster bone degradation，which can be better utilized for Beagles’ lateral femoral condyle B1 fractures. The mechanism for Citric acid material to promote fracture healing may be related to the TCA cycle.

Key words: Biodegradable bone screws；　Lateral femoral epicondyle fracture；　Citric acid； , Imaging； , , Histology

CLC Number:

R318.08

Li Zhen, Huang Hai, Liao Jianwen, Fan Shicai, Yang Cheng. Imaging and histology research of citrate-based biodegradable screws [J]. Chinese Journal of Clinical Anatomy, 2021, 39(1): 60-64.

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Imaging and histology research of citrate-based biodegradable screws

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