Abstract: Objective　To inhibit the expression of IL-10 and to observe IL-10 affected the recovery of motor function of spinal cord contusion rats by glyoxalase-1 by using lentivirus mediated RNA.　Methods　The model of spinal cord contusion (SCC) was established in 66 Sprague-Dawley rats with Allen's method. The rats were randomized into a Sham group (n=9); a SCC group (n=27)、a Vector group (n=15) and a IL-10 SH group (n=15), which were injected blank lentivirus vector and vector of IL-10 siRNA respectively and then divided into 3 days, 7 days and 28 days subgroups. Basso, Beattie, Bresnahan (BBB) score was used to evaluate the motor function recovery of hind limbs in rats. The localization and expression of GLO1 were examined by immunohistochemistry and real-time quantitative polymerase chain reaction (qRT-PCR) respectively. The inhibition of IL-10 (IL-10-SH-LV) mediated by lentivirus transduction was used to elucidate the function of IL-10 and GLO1. GeneMANIA was used to predict the relationship between IL-10 and GLO1.　Results　The motor capability of hind limbs disappeared after SCC and followed by recovery at 1d, 3d, 7d and 14d as evaluated by BBB score, but it still could not reach the normal level. The expression of IL-10 decreased obviously in spinal cord tissue after SCC was detected by qRT-PCR, and the immunoreactive positive reactant of IL-10 was located in neurons and neuroglial cells of ventral horn in spinal cord by immunofluorescence. Inhibition of IL-10 mediated by lentivirus reduced motor function (evaluated by BBB score), but increased in the next time with the upregulation of GLO1. Futher more, GeneMANIA result showed that GLO1 contacted with IL-10 through Hpd and Klk1c2/Kcns1/Proc, which were co-expression in rats.　Conclusions　IL-10 promotes functional recovery of spinal cord contusion rats, which may be associated with upregulation of GLO1.

Key words: IL-10;　GLO1, 　RNAi;　Rats;　Spinal cord contusion