缺氧诱导因子1-血管内皮生长因子-Notch信号通路参与马蹄内翻足畸形的发生机制

doi:10.13418/j.issn.1001-165x.2022.1.12

Abstract

Abstract: Objective　To study the molecular mechanism of equinovarus deformity.　Methods　HE staining was used to observe the difference of foot and ankle tissue structure in model group and control group. The expressions of hypoxia inducible factor 1 (HIF-1), vascular endothelial growth factor (VEGF) and Notch-1 in the tissues of foot and ankle of rats in model group and control group were detected by immunohistochemical staining, Western Blot and qRT-PCR.　Results　Microscopic observation results showed that compared with the control group, the tissue structure of the foot and ankle in the experimental group was relatively disordered, with enlarged tissue space, loose soft tissue and aggregation contracture of local soft tissue. Immunohistochemical staining, Western Blot and qRT-PCR results showed that compared with the control group, the expression of HIF-1 down-regulated in ankle tissue of the experimental group, and the expression of VEGF and Notch-1 decreased.　Conclusions 　HIF-VEGF-Notch signaling pathway may be related to the occurrence of varus foot deformity.

Key words: Hypoxia inducible factor 1 (HIF1), 　Vascular endothelial growth factor (VEGF), 　Notch signaling pathway, 　Congenital talipes equinovarus

CLC Number:

Q291

Zhang Zhenyi, Chen Xi, Wang Qian. HIF-VEGF-Notch signaling pathway may be involved in the occurrence of equinovarus deformity[J]. Chinese Journal of Clinical Anatomy, 2022, 40(1): 62-66.

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HIF-VEGF-Notch signaling pathway may be involved in the occurrence of equinovarus deformity

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