冻融循环及物理匀浆制备高质量脂肪脱细胞基质

doi:10.13418/j.issn.1001-165x.2018.04.008

Abstract

Abstract:

Objective　Decellularized adipose tissue (DAT) was prepared by physical homogenization to improve its ability of spontaneous lipid induction.　Methods　In this study, we used homogenization method to remove lipid from adipose tissue, a novel DAT (DAT-1) was fabricated after subsequent decellularization process. Ordinary DAT served as control.　Results　The results revealed that DAT-1 achieved the same decellularization efficiency, but exhibited better retention of ECM components (laminin and collagen Ⅳ) and in vivo recellularization, compared to those with ordinary DAT.　Conclusions 　Mechanical homogenization is a rapid and efficient method to remove lipid from adipose tissue, which could avoid the use of harsh chemical agent and lead to the better retention of ECM components, promoting the in vivo adipose tissue regeneration of DAT.

Key words: Decellularized matrix, 　Freeze-thaw cycle, 　Mechanical homogenization, 　Adipose tissue regeneration

PENG Zhang-song, HE Yun-fan, LU Feng. Prepare decellularized adipose tissue by freeze-thaw cycle and mechanical homogenization[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(4): 392-396.

References

[1] 廖选，刘宏伟. 脂肪组织脱细胞基质研究现状及展望[J]. 中国美容整形外科杂志, 2017, 28(12): 729-732.
[2] Adam Young D, Bajaj V, Christman KL. Award winner for outstanding research in the PhD category, 2014 Society for Biomaterials annual meeting and exposition, Denver, Colorado, April 16-19, 2014: Decellularized adipose matrix hydrogels stimulate in vivo neovascularization and adipose formation [J]. J Biomed Mater Res A. 2014, 102(6): 1641-1651.
[3] Kawecki M, ?abu? W, Klama-Baryla A，et al. A review of decellurization methods caused by an urgent need for quality control of cell-free extracellular matrix' scaffolds and their role in regenerative medicine[J]. J Biomed Mater Res B Appl Biomater. 2018, 106(2): 909-923.
[4] Banyard DA, Borad V, Amezcua E, et al. Preparation, Characterization, and Clinical Implications of Human Decellularized Adipose Tissue Extracellular Matrix (hDAM): A Comprehensive Review[J]. Aesthet Surg J, 2016, 36(3): 349-357.
[5] Yao Y, Dong Z, Liao Y, et al. Adipose extracellular matrix/stromal vascular fraction gel:a novel adipose tissue-derived iInjectable for stem cell therapy[J]. Plast Reconstr Surg, 2017, 139(4): 867-879.
[6] Flynn LE. The use of decellularized adipose tissue to provide an inductive microenvironment for the adipogenic differentiation of human adipose-derived stem cells[J]. Biomaterials, 2010, 31(17): 4715-4724.
[7] Keane TJ, Swinehart IT, Badylak SF. Methods of tissue decellularization used for preparation of biologic scaffolds and in vivo relevance[J]. Methods, 2015, 84: 25-34.
[8] 彭蒙蒙，梅劲. 器官脱细胞化的研究进展[J]. 中国临床解剖学杂志, 2012, 30(3): 362-364.
[9] Banyard DA, Borad V, Amezcua E, et al. Preparation, characterization, and clinical implications of human decellularized adipose tissue extracellular matrix (hDAM): a comprehensive review[J]. Aesthet Surg J, 2016, 36(3): 349-357.
[10]Flynn L, Semple JL, Woodhouse KA. Decellularized placental matrices for adipose tissue engineering[J]. J Biomed Mater Res A, 2006, 79(2): 359-369.
[11]Rieder E, Kasimir MT, Silberhumer G, et al. Decellularization protocols of porcine heart valves differ importantly in efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells[J]. J Thorac Cardiovasc Surg, 2004, 127(2): 399-405.
[12]O'Neill JD, Anfang R, Anandappa A, et al. Decellularization of human and porcine lung tissues for pulmonary tissue engineering [J]. Ann Thorac Surg, 2013, 96(3): 1046-1056.
[13]Tapias LF, Ott HC. Decellularized scaffolds as a platform for bioengineered organs[J]. Curr Opin Organ Transplant, 2014, 19(2): 145-152.
[14] He Y, Lin M, Wang X, et al. Optimized adipose tissue engineering strategy based on a neo-mechanical processing method[J]. Wound Repair Regen. 2018, 26.
[15] Kuljanin M, Brown CFC, Raleigh MJ, et al. Collagenase treatment enhances proteomic coverage of low-abundance proteins in decellularized matrix bioscaffolds[J]. Biomaterials, 2017, 144: 130-143.

Prepare decellularized adipose tissue by freeze-thaw cycle and mechanical homogenization

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Metrics

Comments

Recommended 0