VIP对LPS应激的小鼠成纤维细胞TREM-2表达的影响及其机制

doi:10.13418/j.issn.1001-165x.2018.04.010

Abstract

Abstract:

Objective　To observe the effect of VIP(vasoactive intestinal peptide) on TREM-2 expression in mouse fibroblasts induced by LPS(lipopolysaccharides) and to explore its possible signaling pathway. Methods　ALI animal model was established by intraperitoneal injection of LPS, and VIP lentivirus tracheal infusion was used, and the expression of TREM-2 in lung tissue was detected by qPCR. qPCR and flow cytometry were used to detect the effect of VIP on TREM-2 expression in LPS-induced fibroblasts, and to observe the effects of PKC signal pathway blocker (H-7), PKA signal pathway blocker (H-89), MAPK signal pathway blocker (PD98059) and CaM signal pathway blocker (W-7) on the regulation of VIP expression TREM-2.　Results　The TREM-2 mRNA expression of lung tissue in mice decreased in ALI, while VIP could increase TREM-2 mRNA expression in lung tissue. LPS down-regulated the expression of TREM-2 mRNA in mice fibroblast, and VIP up-regulated the expression of TREM-2 mRNA in a time-dependent manner (0 h, 3 h, 6 h, 12 h, 24 h), and reached the peak at 6 h. VIP dose related with the TREM-2 mRNA expression (10-10 mol/L, 10-9 mol/L, 10-8 mol/L, 10-7 mol/L). The up-regulation effect of VIP on TREM-2 mRNA and protein expression in mouse fibroblasts (stress by LPS for 6 h) could be blocked by H-7, PD98059, and W-7. 　Conclusion　LPS can down-regulate the TREM-2 expression of mouse fibroblasts, while VIP can up-regulate the expression of TREM-2 in LPS induced fibroblasts, and the intracellular signal transduction pathway may be related to PKC, MAPK and CaM.

Key words: Acute lung injury;　Triggering receptor expressed on myeloid cells-2;　Vasoactive intestinal peptide;　Lipopolysaccharide; , Fibroblast

LI Shu-fen, SONG Zhuo-hui, YANG Hui-hui, LIU Yong-ping, XIONG Jian-bing, GUAN Cha-xiang, SUN Guo-ying. Effect of VIP on the expression of TREM-2 in mouse fibroblasts with LPS stress and its mechanism[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(4): 402-407.

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Effect of VIP on the expression of TREM-2 in mouse fibroblasts with LPS stress and its mechanism

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