Abstract:

Objective    to optimize the method of paraffin-embedded fine puncture tissue, and improve the quality of paraffin embedding.    Methods    The conditions including embedding molds at different temperatures, adding water or not at the cold plate, different embedding angles, and different secondary wax injection angles were tried, and the embedding acceptability, embedding time, tissue structure integrity, wrinkles, knife marks, cell display effect, and reticular fiber staining effect for small puncture tissues were analyzed and compared.   Results   ① The embedding acceptability was significantly higher  using the embedding mold preheated to 66 ℃ than that at room temperature（χ2=5.26，P＜0.05）. The integrity of histological structure, cell transparency, contrast and clarity of nucleoplasm and reticular fiber integrity were all better than normal temperature group (P＜0.05). ② Both embedding acceptability and efficiency were better by adding a  small amount of water to the cold plate compared to that without water(P＜0.05).③ Setting the embedding angle (between biopsy tissue and embedding mold) to 30°~45°, slices showed better quality  than the angle set to 0° or 90° (P＜0.05). ④ The embedding acceptability in the embedding mold at a tilt of 10°~25° during secondary wax injection was significantly higher than that at untilted ones（χ2=4.82，P＜0.05）.   Conclusion    By optimizing the conditions including embedding mold temperature, embedding machine cold plate, tissue embedding angle, and secondary wax injection angle can significantly improve the embedding acceptability, embedding efficiency, ensuing slices’quality, cell display effect, and reticular fiber dyeing effect for fine puncture tissues.

Key words: Optimization；  , Fine；    , Puncture tissue；   , Paraffin embedded；   , Quality