还原型谷胱甘肽对活性氧介导的系统性红斑狼疮患者中性粒细胞NETosis的影响

doi:10.13418/j.issn.1001-165x.2018.05.010

Chinese Journal Of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (5): 532-535.doi: 10.13418/j.issn.1001-165x.2018.05.010

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The effect of reduced glutathione on the ROS-mediated NETosis by neutrophils from patients with systemic lupus erythematosus

LIAO Pan ^1,2，HE Yi ^1,2，YANG Fang-yuan ^1,2，ZHUANG Jian ^1,2，LUO Gui-hu ^1,2，ZHAI Ze-qing ^1,2，SUN Er-wei ^1,2

1. Department of Rheumatology and Immunology, The Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, China; 2. Institute of Clinical Immunology, Academy of Orthopedics, Guangdong Province, Guangzhou 510630, China

Received:2018-07-31 Online:2018-09-25 Published:2018-10-26

Abstract

Abstract:

Objective　Evidence has demonstrated that the formation of neutrophil extracellular traps (NETs) formation mediated by neutrophil plays an important role in the pathogenesis and progression of systemic lupus erythematosus (SLE). In this study, we aimed to investigate whether reduced glutathione (GSH) could decrease the level of intracellular reactive oxygen species (ROS) or inhibit NETs formation. Methods　Neutrophils were isolated from peripheral blood of SLE patients by gradient centrifugation method dextran sedimentation and centrifugation, and then were stimulated with phorbol 12-myristate 13-acetate (PMA). The production of intracellular ROS and the formation of NETs were examined. Results Compared with the PMA group treated with PMA, the group preprocessing with GSH could markedly suppress formation of NET by neutrophils from SLE patients. Moreover, the production of intracellular ROS production was significantly decreased in GSH-treated neutrophils from SLE patients. Conclusion　GSH can significantly inhibit the process of neutrophils' NETosis from SLE patients through modulating the intracellular ROS production. These results indicates that GSH might be a potential therapeutic agent for SLE.

Key words: Reduced glutathione, 　Systemic lupus erythematosus, 　Neutrophils, 　NETosis, 　Reactive oxygen species

LIAO Pan，HE Yi，YANG Fang-yuan，ZHUANG Jian，LUO Gui-hu，ZHAI Ze-qing，SUN Er-wei. The effect of reduced glutathione on the ROS-mediated NETosis by neutrophils from patients with systemic lupus erythematosus[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(5): 532-535.

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The effect of reduced glutathione on the ROS-mediated NETosis by neutrophils from patients with systemic lupus erythematosus

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