鼠李素下调Notch-1的表达对乳腺癌MCF-7细胞增殖、侵袭和迁移的调节作用

doi:10.13418/j.issn.1001-165x.2018.06.007

Abstract

Abstract:

Objective　To explore the effect, and its molecular mechanism, of rhamnetin on cell proliferation, invasion and migration of MCF-7 cell which is a kind of breast cancer cell. Methods　MCF-7 cells were divided into four groups: MCF-7 (control group) and rhamnetin (1, 2.5, 5 μM) groups. Cell proliferaion was detected by CCK-8. Cell invasion was measured by transwell. Cell migration was tested by wound healing. The expression of Notch-1, CSL, Hes1, Ki67 and VEGF were detected by western blot. Results Low concentration (< 5 μM) rhamnetin had no effect on the cell viability of MCF-7, while it was quite opposite under the circumstance of high concentration (> 5 μM) rhamnetin. The proliferation in rhamnetin (2.5, 5 μM) groups were lower than that of control group (P<0.05). Compared with control group, the invasion and migration in rhamnetin (1, 2.5, 5 μM) groups were decreased (P<0.05) with lower expression of Notch-1, CSL and Hes1 in rhamnetin (P<0.05). Moreover, the rhamnetin (5 μM)-induced declined expression of Notch-1, CSL, Hes1, Ki67 and VEGF were repressed by Notch-1 pathway activating agent Jagged1 (10 μM). Conclusions　Rhamnetin can restrain the proliferation, invasion and migration of MCF-7 via down-regulating the expression of Notch-1.

Key words: Breast cancer; , Rhamnetin; , Proliferation; , Invasion; , Migration; , Notch-1 pathway

ZHANG Shu-yan1, ZHANG Fang1, JI Ying-hua2. Rhamnetin regulates the proliferation, invasion and migration of breast cancer MCF-7 via down-regulating the expression of Notch-1[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(6): 632-636.

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Rhamnetin regulates the proliferation, invasion and migration of breast cancer MCF-7 via down-regulating the expression of Notch-1

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