帕金森病猴模型生化指标及肾上腺的改变

doi:10.13418/j.issn.1001-165x.2019.01.011

Abstract

Abstract:

Objective　To investigate the changes of biochemical indicators and adrenal glands after chronic MPTP treatment in cynomolgus monkeys. Methods　Eight male cynomolgus monkeys aged about 10 years old were randomly divided into two groups: a control group (n=3) and a model group (n=5) receiving MPTP administration for 30 days. The animal behavior was observed and recorded during animal modelling. After successful cynomolgus monkey modelling, blood was drawn to measure the kidney function, glucose, cholesterol, and triglycerides. After anaerobic animal anesthesia, the adrenal gland was taken for HE, α-synuclein, dopamine receptor D2 staining and pathological changes were observed.　Results　The PD model was successfully established in cynomolgus monkeys. There were significant differences in the biochemical indexes of glucose(GLU) and cholesterol(CH) between the groups (P<0.05). There was no significant difference in renal function. The adrenal gland was damaged. The inflammatory cells infiltrated. The boundary between the capsule and medulla was basically unclear. The cortical layer became thinner, and some of it underwent focal necrosis. The expression of α-synuclein in the adrenal gland was significantly higher than that in the control group, while the dopamine receptor D2 in the medulla was significantly reduced. There were statistically significant differences between the two groups in immunohistochemical semi-quantitative analysis(P<0.05).　Conclusions　The metabolic index of cynomolgus PD model is significantly decreased. The structural function of adrenal gland may be related to the pathogenesis of PD. The detection of peripheral biological indicators provides a new idea for the diagnosis of PD.

Key words: Parkinson's disease； , Adrenal gland；　Renal function；　Dopamine receptor, α-synuclein

TIAN Jing-hua，ZHU Ming-hui，ZHANG Zheng-hui， YU Lan. The changes of adrenal gland and biochemical indicators in cynomolgus monkey models of Parkinson’s disease[J]. Chinese Journal Of Clinical Anatomy, 2019, 37(1): 51-54.

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The changes of adrenal gland and biochemical indicators in cynomolgus monkey models of Parkinson’s disease

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