骨髓间充质干细胞和心脏Sca1 阳性细胞移植治疗心肌梗死的效果比较及其机制研究

doi:10.13418/j.issn.1001-165x.2019.02.009

Abstract

Abstract:

　Objective　To investigate the therapeutic effect of bone marrow mesenchymal stem cell (MSCs) and cardiac-derived Sca1 positive stem cell on acute myocardial infarction model in mice.　Methods　Bone marrow mesenchymal stem cells were isolated from the femur bone marrow of adult C57BL/C mice, and Sca1 positive stem cells were harvested from the heart by flow cytometry. Twenty-four male C57BL/C mice were divided into four groups: sham group, control group, bone marrow MSCs treated group and Sca1 positive cells treated group. After anesthesia, the mouse thorax was opened and the left anterior descending coronary artery (LAD) was ligated to establish the acute myocardial infarction model. After successful LAD ligation, cells were injected into the infarcted myocardium. Four weeks later, cardiac ultrasound was used to observe heart function: the left ventricular ejection fraction (LVEF) and left ventricular (LV) end dilated volume. In addition, quantitative PCR was used to evaluate the RNA expression of VEGFa and VEGFb in cultured cells.　Results　The cultured bone marrow MSCs adhered to the dish and showed a spindle shape. The positive rate of Sca1 positive stem cells was about 18 %, and they also adhered to the dish while showed a short shuttle-like shape. Compared with Sca1positive stem cell therapy, MSCs treatment increased the survival rate of mice after myocardial infarction. After 4 weeks of cell transplantation therapy, the LVEF of the bone marrow-derived MSCs treatment group was significantly higher than that of the cardiac-derived Sca1 positive stem cell treatment group, while the LV end dilated volume was lower. The expression of VEGFa and VEGFb was significantly higher in bone-derived MSCs. 　Conclusion　Both bone marrow-derived MSCs and Cardiac-derived Sca1positive stem cells are beneficial to the improvement of cardiac function after infarction, and bone marrow-derived MSCs are superior in improving LVEF and inhibiting enlargement of left ventricular after myocardial infarction in mice, which may be caused by the high expression of VEGF in bone marrow derived MSC.

Key words: Bone marrow mesenchymal stem cell, Sca1; , Myocardial infarction; , Cell transplantation; , VEGF

SUN Qi, SHAN Wei. Comparison of the effect and mechanism of transplantation of bone marrow mesenchymal stem cells and cardiac sca1-positive stem cells on myocardial infarction[J]. Chinese Journal Of Clinical Anatomy, 2019, 37(2): 160-164.

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Comparison of the effect and mechanism of transplantation of bone marrow mesenchymal stem cells and cardiac sca1-positive stem cells on myocardial infarction

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