Effect of genistein on the number and function of HUVECs in preeclampsia patients and its mechanisms

doi:10.13418/j.issn.1001-165x.2019.03.005

Chinese Journal of Clinical Anatomy ›› 2019, Vol. 37 ›› Issue (3) : 261-266. DOI: 10.13418/j.issn.1001-165x.2019.03.005

LIU Li-xing¹ , MIN Xian-hui ², ZHANG Min¹, HOU Sai-rong¹

Author information +

History +

Abstract

Objective　To study the effect of genistein on endothelial cell damage in preeclampsia. Methods　Human umbilical vein cells (HUVECs) were isolated from 20 normal pregnant women (control group) and 40 preeclampsia patients. Half of cells from the preeclampsia patients were treated with genistein (PE+Gen group) and the other half were untreated (PE group). HUVEC barrier function and proliferation were investigated using bovine serum albumin and MTT assays, respectively. Markers for oxidative stress and apoptosis were also assessed. 　Results　Compared to the PE group, HUVEC barrier function, proliferation, glutathione peroxidase activity, and Bcl-2 gene and protein expression were significantly increased in the genistein treatment group (PE+Gen), whereas 8-hydroxy-2’-deoxyguanosine levels and Bax gene and protein expression were significantly decreased (P<0.01). There was no significant difference between PE+Gen and control groups.　Conclusion　Genistein reduces endothelial cell damage possibly through antioxidant and anti-apoptotic mechanisms, and it may be useful in preeclampsia prevention and treatment.

Key words

/ Genistein / Preeclampsia / 　Human umbilical vein endothelial cells / 　Oxidative stress / Anti-apoptosis

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LIU Li-xing , MIN Xian-hui , ZHANG Min, HOU Sai-rong. Effect of genistein on the number and function of HUVECs in preeclampsia patients and its mechanisms[J]. Chinese Journal of Clinical Anatomy. 2019, 37(3): 261-266 https://doi.org/10.13418/j.issn.1001-165x.2019.03.005

References

[1] 乐杰, 妇产科学[M]. 北京: 人民出版社，2008: 92-101
[2] Ramos JGL, Sass N, Costa SHM, Preeclampsia[J]. Rev Bras Ginecol Obstet, 2017, 39(9): 496-512.
[3] Ganai AA, Farooqi H, Bioactivity of genistein: a review of in vitro and in vivo studies[J]. Biomed Pharmacother, 2015, 76: 30-38.
[4] Irace C, Marini H, Bitto A, et al. Genistein and endothelial function in postmenopausal women with metabolic syndrome[J]. Eur J Clin Invest, 2013, 43(10): 1025-1031.
[5] Yan Feng-ying, Yu Ping, Guan Lin-bo, et al. Isolation and identific-ation of primary human umbilical vein endothelial cells[J]. West China Medical Journal, 2010, 25(4): 727-729
[6] 李琴山, 冯赞杰, 刘洋, 等. 一种改进的人脐静脉内皮细胞的培养方法[J]. 第四军医大学学报, 2007, 28(3): 276-278．
[7] Poston L. Endothelial dysfunction in pre-eclampsia[J]. Pharmacol Rep, 2006, 58: 69-74.
[8] El-Sayed AAF. Preeclampsia: a review of the pathogenesis and possible management strategies based on its pathophysiological derangements[J]. Taiwan J Obstet Gynecol, 2017, 56(5): 593-598.
[9] Baker AM, Klein RL, Moss KL, et al. Maternal serum dyslipidemia occurs early in pregnancy in women with mild but not severe preeclampsia[J]. Am J Obstet Gynecol, 2009, 201(3): 293.e1-4.
[10]Liu L, Zhang M, Li L, et al. Expression and clinical significance of ATP-binding cassette transporter 1 in serum and placental tissue in Chinese patients with preeclampsia[J]. Gynecol Obstet Invest, 2014, 78(3): 194-200.
[11] Rodgers GM, Taylor RN, Roberts JM.Preeclampsiais associated with a serum factor cytotoxic to human endothelial cells[J].Am J Obstet Gynecol, 1988 159(4): 908-914.
[12]Perkin AG, Newbury FG. The colouring matters contained in dyer’s broom (Genista tinctoria) and heather (Calluna vulgaris)[J]. J Chem Soc, 1899, 75: 830-839.
[13]Tůmová L, Tůma J. The effect of UV light on isoflavonoid production in Genista tinctoria culture in vitro[J]. Acta Physiol Plant, 2011, 33(2): 635-640.
[14]Szkudelska K, Nogowski L. Genistein–a dietary compound inducing hormonal and metabolic changes[J]. J Steroid Biochem Mol Biol, 2007, 105(1-5): 37-45.
[15]李冬花, 黄红林. 染料木素心血管保护作用及机制研究[J]. 临床与病理杂志, 2015(8): 1551-1558.
[16] 李开莹, 黄远英, 柴金珍, 等. 大豆异黄酮对人体健康的作用[J]. 食品安全导刊, 2016(12): 86-88.
[17]Zhao C, Meng F, Geng L, et al. Cardiac-protective effects and the possible mechanisms of alatamine during acute myocardial ischemia[J]. Can J Physiol Pharmacol, 2016, 94(4): 433-440.
[18]吴利平. 氧化应激与心血管疾病关系的研究进展[J]. 中国处方药, 2017, 15(7): 9-10.
[19]Susnow N, Zeng L, Margineantu D, et al. Bcl-2 family proteins as regulators of oxidative stress[J]. Semin Cancer Biol, 2009, 19(1): 42-49.
[20] Vijayalakshhmi B, Sesikeran B, Udaykumar P, et al. Effects of vitamin restriction and supplementation on rat intestinal epithelial cell apoptosis[J]. Free Ｒadic Biol Med, 2005, 38(12): 1614-1624．
[21] 何玲玲, 封锦芳, 肖荣, 等. 染料木黄酮对β淀粉样肽25-35介导的 PC12 细胞凋亡过程相关基因表达的影响[J]. 中国食品卫生杂志, 2011, 23(2): 100-105．
[22] Maged AM, Aid G, Bassiouny N, et al. Association of biochemical markers with the severity of pre-eclampsia[J]. Int J Gynaecol Obstet, 2017, 136(2): 138-144.
[23] Henderson JT, Thompson JH, Burda BU, et al. Preeclampsia screen-ing: evidence report and systematic review for the US preventive services task force[J]. JAMA, 2017, 317(16): 1668-1683.