Genistein对子痫前期患者离体脐静脉内皮细胞的保护作用及其机制研究#br#

doi:10.13418/j.issn.1001-165x.2019.03.005

Abstract

Abstract: Objective　To study the effect of genistein on endothelial cell damage in preeclampsia. Methods　Human umbilical vein cells (HUVECs) were isolated from 20 normal pregnant women (control group) and 40 preeclampsia patients. Half of cells from the preeclampsia patients were treated with genistein (PE+Gen group) and the other half were untreated (PE group). HUVEC barrier function and proliferation were investigated using bovine serum albumin and MTT assays, respectively. Markers for oxidative stress and apoptosis were also assessed. 　Results　Compared to the PE group, HUVEC barrier function, proliferation, glutathione peroxidase activity, and Bcl-2 gene and protein expression were significantly increased in the genistein treatment group (PE+Gen), whereas 8-hydroxy-2’-deoxyguanosine levels and Bax gene and protein expression were significantly decreased (P<0.01). There was no significant difference between PE+Gen and control groups.　Conclusion　Genistein reduces endothelial cell damage possibly through antioxidant and anti-apoptotic mechanisms, and it may be useful in preeclampsia prevention and treatment.

Key words: , Genistein, Preeclampsia, 　Human umbilical vein endothelial cells, 　Oxidative stress, Anti-apoptosis

CLC Number:

R765.21

LIU Li-xing , MIN Xian-hui , ZHANG Min, HOU Sai-rong. Effect of genistein on the number and function of HUVECs in preeclampsia patients and its mechanisms[J]. Chinese Journal of Clinical Anatomy, 2019, 37(3): 261-266.

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Effect of genistein on the number and function of HUVECs in preeclampsia patients and its mechanisms

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