Abstract: Objective　To discuss the effect of miR-146 and its target genes IRAK1 and TRAF6 on regulating of hyperthyroid cardiopathy. 　Methods　Male C57BL/6J mice (n=60) were randomly divided into a Control group (n=30) and a T4 group (n=30). Mice in the T4 group were intraperitoneally injected with T4 diluent (1 μg/g). Mice in the control group were intraperitoneally injected with normal saline (150 mL). After 7 weeks, electrocardiogram was made after anesthetization; ELISA was used to detect serum TT3 and TT4; HE and Masson stain was used to observe the heart morphological change. qPCR was used to detect the expression of miR-146. Western blot was used to detect the protein level of IRAK1 and TRAF6.　Results　Compared with the Control group, heart rate was significantly increased and electrocardiogram was abnormal in mice of the T4 group; Serum TT3 and TT4 were significantly increased (P<0.001). Cardiomyocytes was disordered in structure and more abundant in collagen fiber; miR-146a and miR-146b were significantly up-regulated, P<0.001; IRAK1 and TRAF6 were significantly down-regulated, P<0.001.  Conclusion　miR-146 may regulate hyperthyroid cardiopathy by IRAK1 and TRAF6.

Key words: Hyperthyroid cardiopathy, 　microRNA-146, 　IRAK1, 　 TRAF6