Abstract: Objective　To investigate the relationship between miR-143-3p targeting MAPK1 and its effect on proliferation, apoptosis and invasion of human colon cancer cells.　Methods　qRT-PCR was used to detect the effect of transfection and the expression level of MAPK1 mRNA. Double luciferase reporter assay was used to detect the targeting relationship between microRNAs-143-3p and MAPK1. The expression level of MAPK1 protein was detected by Western blot. The proliferation multiple was detected by CCK-8. The proliferation was detected by Hoechst staining. The cell invasion was detected by in vitro invasion test. The expression of Ki67, VEGF，MMP-2 and claspase-3 was detected by Western blot.　Results　miR-143-3p mimic inhibited the expression of MAPK1 mRNA and protein in SW620 cells. miR-143-3p mimic and MAPK1 wild-type reporter plasmid decreased the activity of luciferase significantly. After miR-143-3p mimic transfection, the proliferation and invasion of SW620 cells were significantly decreased, the number of apoptotic cells was significantly increased, the expression of Ki67, VEGF and MMP-2 was significantly decreased, and the expression of cl-caspase-3 was significantly increased. The miR-143-3p mimic alleviated the induction effect of high expression of MAPK1 on proliferation and invasion of SW620 cells and inhibition effect on cell apoptosis.　Conclusion　miR-143-3p can inhibit proliferation and invasion of human colon cancer cells and induce apoptosis through possible targeting inhibition of MAPK1.

Key words: Colon cancer, 　Micro RNA, 　MAPK1, 　Proliferation, 　Apoptosis, 　Invasion