内源性雌激素调控脓毒症小鼠心肌L型钙离子通道α1C亚基的表达

doi:10.13418/j.issn.1001-165x.2019.05.009

Abstract

Abstract: Objective　To investigate the effect of endogenous estrogen on myocardial L-type calcium channel α1C subunit (CACNA1C) in septic mice.　Methods　Female Kunming mice underwent bilateral ovarian ablation, a model of sepsis was established by cecal ligation puncture (CLP) 30 days after surgery. The experimental mice were divided into 5 groups: sepsis group (S group), ovariectomy+sepsis group (QS group), sham group (C group), ovariectomy+sham group (QC group), ovariectomy+estradiol benzoate (QE group). The expression levels of left ventricular CACNA1C were detected by real-time PCR and Western blot at 3 h, 6 h, 12 h, and 24 h after CLP. The expression and distribution of CACNA1C was detected by immunohistochemistry 3 h after CLP.　Results　The expression of CACNA1C in QC group was significantly higher than that in the C group, while the expression level of CACNA1C in the QE group was significantly lower than that in the QC group. At 3 h after CLP, the expression of CACNA1C in the S group was significantly higher than that in the control and other time points. At 3 h, 6 h, 12 h and 24 h after CLP, the expression of CACNA1C in the QS group was significantly lower than that in the control group, and there was no significant difference among the four time points.　Conclusion The expression of CACNA1C is significantly increased after bilateral ovarian ablation. Replenishment of estradiol benzoate after lateral ovarian ablation can reverse the increase of CACNA1C. The expression of CACNA1C is transiently increased at 3 h after CLP in normal mice, while the expression of CACNA1C at each time point after CLP in bilateral ovarian-extracted mice is significantly lower than that in the control group.

Key words: , Estrogen；　L- type calcium channel；　CACNA1C；　Sepsis

CLC Number:

R363.2

FENG Ying , LIU Bo-yi, FANG Zhi-cheng, CHEN Li, CHEN Wei, ZHENG Xiang. Endogenous estrogen regulates the expression of myocardial L-type calcium channel α1C subunit (CACNA1C) in septic mice[J]. Chinese Journal of Clinical Anatomy, 2019, 37(5): 523-527.

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Endogenous estrogen regulates the expression of myocardial L-type calcium channel α1C subunit (CACNA1C) in septic mice

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