miR-128-3p过表达靶向MAPK1抑制膀胱癌5637细胞株的侵袭，迁移和上皮间质转化

doi:10.13418/j.issn.1001-165x.2019.05.011

Abstract

Abstract: Objective　To investigate the effects of miR-128-3p overexpression on invasion, migration and epithelial-mesenchymal transition of bladder cancer 5637 cells.　Methods　The target gene of miR-128-3p was screened by the gene prediction software TargetScan. And the luciferase reporter assay was verified. The expression of miR-128-3p and MAPK1 was detected by RT-PCR. Cell invasion was detected by Transwell. Cell migration ability was measured by a scratch test. The expressions of E-cadherin, N-cadherin, ERK1/2, c-Myc and c-fos were detected by Western blot, and the expression of Vimentin was detected by immunofluorescence. The xenograft model was established by subcutaneous injection in nude mice. The tumor weight was measured 30 days later. And the survival curve was drawn. The amount of Vimentin, miR-128-3p, MAPK1, ERK1/2, c-Myc and c-fos in the transplanted tumor was detected.　Results　miR-128-3p had been targeted to inhibit the expression of MAPK1. After overexpression of miR-128-3p, the number of invasive cells and wound healing rate was decreased. E-cadherin expression was up-regulated. N-cadherin expression was down-regulated. Vimentin positive was decreased. The expression of ERK1/2, c-Myc and c-fos was down-regulated. Through the intervention of miR-128-3p, the weight of transplanted tumors was decreased. The survival rate of nude mice was increased. The expression of miR-128-3p was up-regulated. The expression of MAPK1 was down-regulated. The expression of Vimentin was decreased. And the expression of ERK1/2, c-Myc and c-fos was decreased.　Conclusion　Overexpression of miR-128-3p inhibits the invasion, migration, epithelial-mesenchymal transition and ERK1/2, c-Myc and c-fos pathways of bladder cancer cell line 5637 by targeting inhibition of MAPK1 expression.

Key words: miR-128-3p, MAPK1, 　Bladder cancer cell line 5637, 　Invasion, 　Migration, 　Epithelial-mesenchymal transition

CLC Number:

R734.2

ZHAO Yang, WAN Yin-xu, CHE Ji-zhong, XU Yan-kai, LI Qing-yuan. Overexpression of miR-128-3p inhibits cell invasion, migration and epithelial-mesenchymal transition by targeting MAPK1 in bladder cancer cell line 5637[J]. Chinese Journal of Clinical Anatomy, 2019, 37(5): 534-541.

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Overexpression of miR-128-3p inhibits cell invasion, migration and epithelial-mesenchymal transition by targeting MAPK1 in bladder cancer cell line 5637

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