EPO对大鼠肠缺血再灌注后肺损伤保护作用及作用机制的研究

doi:10.13418/j.issn.1001-165x.2019.05.012

Abstract

Abstract: Objective　To investigate the protective effects of erythropoietin (EPO) on lung injury after rat intestinal ischemia reperfusion. Methods Thirty male SD rats were divided into 5 random groups (n=6 each): an intestinal ischemia reperfusion group (IIR group), a 1000 U/kg EPO treated (low dosage EPO group), a 3000 u/kg EPO treated (middle dose-EPO group), a 5000 U/kg EPO treated (high dose-EPO group) and a sham-operation group (S group). The intestinal ischemia reperfusion model were established by clamping superior mesenteric artery for 1 hour and reperfusion for half an hour. The three treated groups were administrated with intraperitoneal injection of 1000 U/kg, 3000 U/kg and 5000 U/kg EPO 1 hour before reperfusion. Lung tissues was observed by HE staining. The protein levels of MMP-9 and Caspase-1 in the liver tissues were detected by immunohistochemical staining and Western blot. Results (1) In the IIR group, lung injury was induced by IIR, characterized as histological damage of edema, hemorrhage and neutrophil infilitration, which could be alleviated by EPO. (2)The immunohistochemistry and Western blot results showed that compared with the Sham group, the expression of active MMP-9 and Caspase-1 in the IIR group was increased; after EPO intervention, the MMP-9 and Caspase-1 was decreased. Compared with the L and H groups, the expression of MMP-9 and Caspase-1 in the M group was lower than that of the L and H group (P<0.05). Conclusion　EPO has the protective mechanism on lung damage after rat intestinal ischemia reperfusion. The protective mechanism of EPO may be related to the inhibition of MMP-9 and Caspase-1 which mediated over-inflammation.

Key words: Intestinal ischemia reperfusion; , Lung injury; , , EPO; , , MMP-9; , , Caspase-1

CLC Number:

R363.1

XIAO Li, CHEN Xiao-qin, SHI Qing-ming, WANG Jing , NIE Zheng, LI Jian. Studies on the protection and effect of erythropoietin on lung damage after rat intestinal ischemia reperfusion [J]. Chinese Journal of Clinical Anatomy, 2019, 37(5): 542-546.

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Studies on the protection and effect of erythropoietin on lung damage after rat intestinal ischemia reperfusion

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