Abstract: Objective　To explore the method of constructing the primate model combined with disease of endometriosis and syndrome.　Methods　(1) Six female cynomolgus monkeys were randomly divided into a modeling group (n=5) and a control group (n=1). The modeling group was treated with transabdomal cervical cerclage while the control group was only observed through an open surgery. (2) The blood perfusion of the tongue was measured by PeriCam PSI perfusion speckle imager before the first modeling operation, at the modeling month and after the first, second and third months of modeling. (3) Laparoscopic exploration was performed three months after  the operation. Since no endometriosis lesions were found in the pelvis of the monkey, we changed the modeling method: the monkeys were re-assigned into the modeling group (n=4) and the control group(n=2). In the modeling group, the endometrial tissue fragments were collected during the menstrual period and intraperitoneal injection, and immunosuppressive agents were administered for three months.　Results:　(1) In the laparoscopy after three months of cervical cerclage, no endometriosis lesion was seen in the two groups. (2) The blood perfusion in the monkeys was significantly higher in the modeling group than that in the control group at 2 months after the operation (P<0.05).(3) The trend of blood perfusion  in the surface and base of the tongue of each monkey was consistent.(4) Three months after the intraperitoneal injection with the immunosuppressive agents, the modeling group had severe pelvic adhesions, vascular hyperplasia and hyperemia on the serosa of the uterine under the laparoscopy. The endometriosis lesions were found in one of the modeling monkeys.  Conclusion  The endometrial pelvic injection with the administration of the immunosuppressive agents is a feasible approach for constructing primate endometriosis models. The method of measuring the blood perfusion of the tongue of the cynomolgus monkey using PSI is easy and stable, and it may become a quantitative evaluation method for the tongue image of the disease syndrome combination model.

Key words: Endometriosis, 　Cynomolgus monkey model, 　PeriCam PSI blood perfusion speckle imager, 　Tongue image, 　Microcirculation