Abstract: Objective　To verify the reliability of the sophisticated eardrum-ossicular chain model and provide theoretical basis and reference for the follow-up experiments by using finite element method to analyze the harmonic response.　Methods　A sophisticated eardrum-ossicular chain model by multi-software synthesized was established, by using Micro-CT and finite element method to obtain the fault data (screw pitch:0.24mm, total cross sectional image: 464 slices). The fault data were compared with the data which obtained from the temporal bone specimen scanned by laser doppler.　Results　The amplitudes of the tympanic membrane data collection points were 0.022, 0.031 and 0.0041 microns at frequency of 100Hz, 800Hz, 8000Hz respectively, and the amplitudes of the stapes plate data collection points were 0.011, 0.015 and 0.000069 microns respectively. The result of the models’ harmonic response was consistent with the result of laser doppler.　Conclusions　We can rebuild effectively a sophisticated eardrum-ossicular chain by establishing the model, we can use this model to analyze physiological function and pathologic change of the ossicular chain.

Key words: Eardrum-ossicular chain model, 　FEM, 　Biomechanics, 　Harmonic response analysis, Digital reconstruction