miR-21 alleviates the cytotoxicity of mutant huntingtin through PTEN/AKT

doi:10.13418/j.issn.1001-165x.2020.01.011

Chinese Journal of Clinical Anatomy ›› 2020, Vol. 38 ›› Issue (1) : 51-56. DOI: 10.13418/j.issn.1001-165x.2020.01.011

LI Xi-fan¹, CHEN Tian¹, FANG Fang¹, LI He²

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Abstract

Objective　To detect the mechanism of miRNA-21 in reducing cytotoxicity of mutant Huntingtin (mHtt).　Methods　The changes of miRNA-21 in brain tissues of Huntington's disease (HD) transgenic mice and HEK293-160Q cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR) ; Whether PTEN was the target gene of miRNA-21 or not was identified by double luciferase test; after transfected the miRNA-21 mimics, cell viability was detected by CCK8, caspase-3 activity was determined by caspase-3 activity assay kit; PTEN, phosphorylated-Ser-473 AKT and AKT were detected by Western Blotting.　Results　qRT-PCR result showed that the levels of miRNA-21 in brain tissues of HD transgenic mice and HEK293-160Q cells were significantly decreased compared with wild-type mice and HEK293-20Q cells; PTEN was the target gene of miRNA-21 which was confirmed by double luciferase test; Western Blotting showed that PTEN decreased significantly and p-AKT-Ser 473 AKT/AKT significantly increased in HEK293-160Q cells after transfected the miRNA-21 mimics.　Conclusions　miR-21 can increase the p-AKT-Ser 473/AKT by decreasing the PTEN, increase the cell viability and inhibit the apoptosis by alleviating the cytotoxicity of mHtt.

Key words

Mutant huntingtin / 　miR-21 / 　PTEN / 　AKT

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LI Xi-fan, CHEN Tian, FANG Fang, LI He. miR-21 alleviates the cytotoxicity of mutant huntingtin through PTEN/AKT[J]. Chinese Journal of Clinical Anatomy. 2020, 38(1): 51-56 https://doi.org/10.13418/j.issn.1001-165x.2020.01.011

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