蛋白激酶WEE1在胃癌组织中的表达及其临床意义

doi:10.13418/j.issn.1001-165x.2020.06.011

Abstract

Abstract: Objective　To investigate the expression of protein kinase WEE1 in gastric cancer tissues and the association between its protein expression and clinical indicators of patients with gastric cancer, and to clarify its role in the occurrence and development of gastric cancer.　Methods　The Oncomine database was used to analyze the differential expression of WEE1 in gastric adenocarcinoma and gastric mucosa. Tissue microarray and immunohistochemistry were used to detect the expression of WEE1 protein using 100 cases of gastric adenocarcinoma and 80 cases of adjacent tissues. The proportion of staining in immunohistochemistry and the degree of staining were semi-quantitatively analyzed, and the correlation between semi-quantitative analysis and clinical indexes of gastric cancer patients was relevance analyzed. Furthermore, reverse transcription (RT)-polymerase chain reaction (PCR) and Western blotting were used to detect the mRNA and protein expression of WEE1 in gastric cancer cells and gastric mucosa cells.　Results　The positive rate of WEE1 in gastric cancer tissues was 86%, of which 14 cases were low expression and 86 cases were high expression of the 100 patients. Oncomine database analysis showed that the expression of WEE1 mRNA in mixed gastric adenocarcinoma was higher than that in gastric mucosa (P<0.01). The results of immunohistochemistry, RT-PCR and Western blotting showed that the expression of WEE1 in gastric cancer tissues was significantly higher than that in adjacent tissues (P<0.01). Moreover, the immunohistochemistry showed that the protein expression of WEE1 increased with the increasing clinical stage of gastric cancer (P<0.01). 　Conclusions Protein kinase WEE1 is highly expressed in gastric cancer tissues, suggesting that it may act as one of the therapeutic targets of gastric cancer.

Key words: Protein kinase WEE1, 　Gastric cancer, 　Immunohistochemistry, 　Tissue microarray, , Western blotting, , , RT-PCR

CLC Number:

R735.2

LI Jing, JIN Yu, LI Qi-yang, XU Ying-zhuo, LIU Chao, XIAO Jian-ying. Expression of protein kinase WEE1 in gastric cancer tissue and its clinical significance[J]. Chinese Journal of Clinical Anatomy, 2020, 38(6): 680-684.

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Expression of protein kinase WEE1 in gastric cancer tissue and its clinical significance

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