3种成软骨诱导培养基对单层培养和微球培养的人脂肪干细胞成软骨效果比较

doi:10.13418/j.issn.1001-165x.2021.01.014

Abstract

Abstract: Objective　To compare the chondrogenic differentiation of human adipose mesenchymal stem cells (hASCs) in vitro by three components of chondrogenic induction medium.　Methods　hASCs were extracted from human adipose tissues and then performed in monolayer culture and microsphere culture respectively. Three kinds of different components of chondrogenic induction medium (group A: Fetal bovine serum (FBS) + high concentration TGF-β3, group B: Insulin, Transferrin, Selenium Solution (ITS) + low concentration TGF-β3, group C: ITS + high concentration TGF-β3) were added. After 3 weeks, the cell proteins in monolayer culture were extracted for detection of cartilage-related markers, and the cell microspheres were stained with Toluidine blue, Alcian blue, Sirius red to compare the effect of cartilage formation. Results 　After 3 weeks, the expression of cartilage markers was the highest in the group C of monolayer culture system, and the staining of microspheres was also the deepest in the group C.　Conclusions　As a medium additive, ITS has a better effect than FBS in inducing chondrogenic differentiation of hASCs. As an important growth factor stimulating chondrogenesis of hASCs, the concentration of 10 ng/ml TGF-β3 has a stronger effect than the concentration of 1 ng/ml.

Key words: 人脂肪干细胞（hASCs）, 　成软骨分化, 　胎牛血清（FBS）, 　转化生长因子3（TGF-β3）

CLC Number:

R684

Yu Qinghe, Qu Rongmei, Zhang Guowei, Li Xin, Qiu Xianshuai, Ouyang Jun, Dai Jingxing, Min Shaoxiong. Comparison of chondrogenic effects of three kinds of chondrogenic induction media on human adipose-derived stem cells in monolayer and microsphere cultured[J]. Chinese Journal of Clinical Anatomy, 2021, 39(1): 71-75.

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Comparison of chondrogenic effects of three kinds of chondrogenic induction media on human adipose-derived stem cells in monolayer and microsphere cultured

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