颞叶外侧癫痫患者脑组织NHE1及凋亡相关蛋白的表达

doi:10.13418/j.issn.1001-165x.2021.04.013

Abstract

Abstract: Objective　To detect the expression of Na+/H+ exchanger-1 (NHE1) and apoptosis related protein in brain tissue of patients with lateral temporal lobe epilepsy after operation and explore the pathogenic mechanism preliminarily.　Methods　Sixteen samples from patients with lateral temporal lobe epilepsy were collected as an epilepsy group, 10 samples from patients with temporal lobe tumor and cerebral hemorrhage as a control group. The pathological changes of brain tissue were observed by HE staining. The expression of NHE1 were further detected by immunohistochemistry and immunofluorescence. Western blot and other method was used to semi-quantitatively analyze the expression characteristics of NHE1, Bcl-2 and Bax. The data were collected and analyzed by SPSS 24.0 software.　Results　HE staining showed that neurons decreased, phagocytosis increased and microglia was hyperactive in the temporal lobe of the epilepsy group. However, there was no similar expression in the control group. Immunofluorescence and immunohistochemical staining showed that NHE1 was located on neurons. NHE1 staining in the epilepsy group was significantly higher than that in the control group (P<0.05). The expression of NHE1 protein in the epilepsy group was significantly higher than that in the control group (P<0.05). The results of Bcl-2 and Bax showed that the expression of Bcl-2 in the epilepsy group was significantly lower than that in the control group (P<0.05), and the expression of Bax was significantly higher than that in the control group (P<0.05).　Conclusions　NHE1 is closely related to the formation and development of lateral temporal lobe epilepsy. The possible mechanism is closely related the increasing of NHE1 and neuron apoptosis.

Key words: Lateral temporal lobe epilepsy, 　NHE1, 　Apoptosis, 　Human brain tissue

CLC Number:

R742.1

Wu Xuling, Dong Lian, Peng Shuang, Ye Lan, Xu Zucai, Feng Zhanhui. The expression of NHE1 and apoptosis related protein in brain tissue of patients with lateral temporal lobe epilepsy[J]. Chinese Journal of Clinical Anatomy, 2021, 39(4): 431-436.

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The expression of NHE1 and apoptosis related protein in brain tissue of patients with lateral temporal lobe epilepsy

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