Abstract: Objective　To investigate the regulatory effect of anisodamine on brain injury and neurological damage in young rats with hypoxic-ischemic brain damage.　Methods　Fifty young rats were divided into five groups (n=10): a healthy control group, a model group, and three model dosing groups (intravenously injected anisodamine for 2.5, 5, 10 mg/kg). Brain tissue wet and dry weight method was used to detect brain index and brain water content. HE staining was used to observe the pathological damage of brain tissue. TUNEL staining was used to observe the apoptosis of brain tissues around hippocampal neurons. Western blot detects the protein expression levels of Bax/Bcl-2, caspase-9, caspase-3, BDNF and NGF in brain tissue. RT-PCR was used to detect the expression level of BDNF and NGF mRNA, and the kit was used to detect the content of SOD, MDA and GSH-Px.　Results　Compared with the healthy control group, the brain tissue and nerve function of young rats in the model group were seriously damaged (P<0.05). Compared with the model group, the brain index and brain water content of young rats in the 5 mg/kg and 10 mg/kg anisodamine groups significantly reduced (P<0.05). The pathological damage of the brain tissue significantly improved, and the apoptotic cells around the hippocampal neurons in the brain Apoptosis decreased, Bax/Bcl-2, caspase-9, and caspase-3 expression levels decreased (P<0.05), BDNF and NGF expression levels increased (P<0.05), MDA content decreased, SOD and GSH-Px content increased (P<0.05).　Conclusions　Anisodamine can alleviate brain damage and nerve function damage in young rats with HIBD.

Key words:  , Anisodamine, 　Hypoxic-ischemic brain damage, 　Young rats model