新型机械敏感离子通道Piezo1和TRPV4蛋白siRNA双沉默模式对骨关节炎大鼠模型的修复作用研究

doi:10.13418/j.issn.1001-165x.2021.06.014

Abstract

Abstract: Objective To explore the repair effect of siRNA double silencing of new mechanosensitive ion channels Piezo1 and TRPV4 on osteoarthritis rat model. Methods The osteoarthritis animal (OA) model of SD rats was established. According to the different treatment on rats, SD rats were divided into the following four groups: a blank control group, a siRNA-Piezo1 group, a siRNA-TRPV4 group and a double gene silencing group. HE staining and safranin solid green staining were used to evaluate the quality of the samples by modified Minkin's score and OARSI score. The expression of Aggrecan protein and Collagen II in articular cartilage were detected by immunohistochemical staining and RT-PCR. Results The result of the HE staining and safranin green staining showed that the scores of knee cartilage tissue in the siRNA-Piezo1 group and siRNA-TRPV4 group were significantly higher than that in the model group, the siRNA-piezo1 group and the siRNA-TRPV4 group (P<0.05). The results of immunohistochemical staining and RT-PCR showed that the expression of Aggrecan and Collagen II in the double gene silencing group was significantly higher than that in siRNA-piezo1 group and siRNA-TRPV4 group (P<0.05). Conclusions Piezo1 and TRPV4 protein siRNA double silencing mode can promote the expression of Aggrecan and Collagen II, and then play a certain role in the repair of osteoarthritis rat model.

Key words: Mechanical sensing ion channels; , , Osteoarthritis; , , Animal model; , , Repair

CLC Number:

R684.3

Zhou Yongwei, Zhang Caihua, Li Xiaofei, Cai Xunzi. The repair effect of novel mechanical sensing ion channels Piezo1 and TRPV4 protein siRNA double silencing on osteoarthritis rat model[J]. Chinese Journal of Clinical Anatomy, 2021, 39(6): 692-699.

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The repair effect of novel mechanical sensing ion channels Piezo1 and TRPV4 protein siRNA double silencing on osteoarthritis rat model

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