Abstract:  Objective    To screen the fractions with the strongest inhibitory effect on the inflammatory response from the ethyl acetate part of Clerodendrum japonicum (Thunb.) Sweet and the dichloromethane-methanol parts with different elution gradients.    Methods   The MTT method was used to determine the safe dose range of the Clerodendrum japonicum (Thunb.) Sweet ethyl acetate site and different elution gradients of dichloromethane-methanol to RAW264.7 cells, and the Clerodendrum  japonicum (Thunb.) Sweet. ethyl acetate site and different elution gradient dichloride were determined by the ELISA method. The secretion of NO, TNF-a, IL-12, IL-6, and IL-1β were detected in RAW264.7 cells induced by LPS, and screened the fraction with the strongest inhibitory effect on the inflammatory response.   Results   The ethyl acetate parts and fractions of Clerodendrum japonicum (Thunb.) Sweet  were within the concentration range of 0.06~2 mg/mL. The inhibitory effect of the ethyl acetate sections and fractions of Clerodendrum japonicum (Thunb.) Sweet on cell viability was gradually stronger, and the concentration above 0.5 mg/mL had obvious cytotoxicity. Below 0.5 mg/mL could enhance cell viability. The high dose of dichloromethane-methanol elution site could inhibit the release of inflammatory factors IL-12, IL-6, TNF-a, IL-1β, but had no inhibitory effect on the secretion of NO.   Conclusions   The anti-inflammation mechanism of the Clerodendrum japonicum (Thunb.) Sweet ethyl acetate site and the dichloromethane-methanol site with different elution gradients is through the inhibition of the secretion of NO, TNF-a, IL-12, IL-6, IL-1β inflammatory factors of cells, dichloride Methane-methanol (50:1) elution site and dichloromethane-methanol (30:1) elution site have strong anti-inflammatory ability.

Key words: Clerodendrum japonicum (Thunb.) Sweet; ,  , Ethyl acetate site; ,  , Flow part; ,  ,  ,  , RAW264.7; ,  , Anti-inflammatory