白藜芦醇对腔隙性脑梗死大鼠脑损伤的影响

doi:10.13418/j.issn.1001-165x.2022.6.10

Abstract

Abstract: Objective To explore the effects of resveratrol (RES) on brain tissue damage in rats with lacunar infarction (LI). Methods Sixty SD rats were divided into a sham operation group, a model group, a RES group and a combination group (RES+LY294002) averagely. LI models were prepared by sodium laurate method. The nerve function was evaluated by Zea-Longa's method. The cerebral infarction volume was observed by TTC. The levels of oxidative stress and inflammatory factors in brain tissues were detected by enzyme linked immunosorbent assay. The microvessel density was observed by Weidener method. The levels of phosphateidylinositol 3 kinase/serine-threonine kinase (PI3K/Akt) signaling pathways related proteins and vascular endothelial growth factor (VEGF) in brain tissues were detected by Western blotting. Results RES could reduce neurological scores, cerebral infarction volume and levels of MDA, TNF-α, IL-6 and IL-1β in LI rats (P<0.05), increase microvessel density, levels of SOD, CAT, GSH-PX and IL-10, and expressions of VEGF, p-PI3K and p-AKT proteins (P<0.05). Compared with the RES group, the above indexes in combination group showed an opposite trend (P<0.05). Conclusions RES can improve brain damage and angiogenesis in LI rats, and PI3K/Akt signaling pathways participate in this process.

Key words: , Lacunar infarction, , , Resveratrol, , , PI3K/Akt signaling pathway, , , , Angiogenesis

CLC Number:

R743.1

Mi Xiaolu, Li Baiyan, Li Yaqin, Zhu Baoyue, Wang Weizhan. Effects of resveratrol on brain damage in rats with lacunar infarction[J]. Chinese Journal of Clinical Anatomy, 2022, 40(6): 683-688.

References 15

[1]	倪佳颖, 姚丽华, 张荣荣. 白藜芦醇对围绝经期抑郁症模型大鼠血清性激素水平及雌激素受体蛋白表达的影响[J]. 中国临床药理学杂志, 2019, 35(17): 1905-1908. DOI: 10.13699/j.cnki.1001-6821. 2019. 17.020.
[2]	王佳, 汪磊, 恽琪, 等. 白藜芦醇对抑郁模型小鼠认知功能及小清蛋白的影响[J]. 中华行为医学与脑科学杂志, 2018, 27(4): 294-299. DOI: 10.3760/cma.j.issn.1674-6554.2018.04.002.
[3]	张跃奇, 王媛, 张志强, 等. 白藜芦醇对慢性脑缺血大鼠认知损伤和海马MMP-9和TIMP-1表达的影响研究[J]. 重庆医学, 2019, 48(5): 742-747. DOI: 10.3969/j.issn.1671-8348.2019.05.005.
[4]	刘茅茅, 王拥军, 李小玲, 等. 白藜芦醇对腔隙性脑梗死模型急性期的干预研究[J]. 中华老年医学杂志, 2003, 22(9): 564-565. DOI: 10.3760/j:issn:0254-9026.2003.09.020.
[5]	刘茅茅, 李小玲,王拥军, 等.腔隙性脑梗死动物模型的建立[J]. 中华老年心脑血管病杂志, 2003, 5(5): 334-337. DOI: 10.3969/j.issn.1009-0126.2003.05.014.
[6]	涂献坤, 杨卫忠, 石松生, 等. 黄芩苷抑制缺血性脑损伤大鼠脑组织TNF-α和AQP-4表达及减轻脑损伤的研究[J]. 中国药理学通报, 2013, 29(9): 1222-1225. DOI: 10.3969/j.issn.1001-1978.2013.09.010.
[7]	Shi SS, Yang WZ, Chen Y, et al. Propofol reduces inflammatory reaction and ischemic brain damage in cerebral ischemia in rats[J]. Neurochem Res, 2014, 39(5): 793-799. DOI: 10.1007/s11064-014-1272-8.
[8]	张远海. 桃红四物汤对脑缺血再灌注损伤大鼠脑内血管新生及PI3K/AKT信号转导通路的影响[J]. 蚌埠医学院学报, 2017, 42(1): 36-56. DOI: 10.13898/j.cnki.issn.1000-2200.2017.01.010.
[9]	何珂峻, 吴爱群. 天然抗氧化剂白藜芦醇的全合成[J]. 中国食品添加剂, 2016, (6): 60-64. DOI: 10.3969/j.issn.1006-2513.2016.06.003.
[10]	刘东媛, 刘妮英, 汪向红, 等. 白藜芦醇对肿瘤Hela细胞增殖和凋亡作用及影响[J]. 中华实验外科杂志, 2018, 35(5): 909-911. DOI: 10.3760/cma.j.issn.1001-9030.2018.05.034.
[11]	王汝朋, 刘延囡, 李广平. 白藜芦醇与心血管疾病研究进展[J]. 中华老年医学杂志, 2018, 37(6): 709-714. DOI: 10.3760/cma.j.issn.0254-9026.2018.06.025.
[12]	崔玉丰, 张博妍, 张瑞, 等. 白藜芦醇对衰老模型小鼠脑组织形态学和氧化应激水平的影响[J]. 卫生研究, 2013, 42(6): 995-998, 1003. DOI: CNKI: SUN:WSYJ.0.2013-06-023.
[13]	李传文, 张嵘, 侯亮, 等. SIRT1/NF-kB通路参与白藜芦醇改善大鼠脑缺血再灌注损伤炎性反应[J]. 安徽医科大学学报, 2018, 53(1): 6-9. DOI: 10.19405/j.cnki.issn1000-1492.2018.01.002.
[14]	Huang Y, Mao Y, Li H, et al. Knockdown of Nrf2 inhibits angiogenesis by down-regulating VEGF expression through PI3K /Akt signaling pathway in cerebral microvascular endothelial cells under hypoxic conditions[J]. Biochem Cell Biol, 2018, 96(4): 475-482. DOI: 10.1139/bcb-2017-0291.
[15]	王晓荣, 乌云高娃, 赵福全, 等. 丁基苯酞通过激活PI3K/Akt/GSK-3β信号通路及其在局部脑缺血损伤脑梗死中的神经保护作用[J]. 实用医学杂志, 2019, 35(19): 2998-3003. DOI: 10.3969/j.issn.1006-5725. 2019.19.005.

Effects of resveratrol on brain damage in rats with lacunar infarction

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