微小RNA-3651过表达通过介导核因子κB信号通路抑制人舌癌细胞CAL27的生长与侵袭

doi:10.13418/j.issn.1001-165x.2023.2.13

Abstract

Abstract: Objective To explore the mechanism of microRNA (miR)-3651 overexpression on inhibiting the growth and invasion of human tongue cancer CAL27 cells by mediating nuclear factor (NF)-κB signaling pathway. Methods CAL27 cells in logarithmic growth phase were divided into a miR-3651 mimic group, a mimic-NC group and a control group. The level of miR-3651 in each group after transfection was detected by fluorescent quantitative real-time polymerase chain reaction (qRT-PCR). The cell vitality in each group was detected by cell counting kit-8 (CCK-8). The invasion ability of cells in each group was detected by Transwell. The migration ability of cells in each group was detected by scratch assay. The formation of epithelial-mesenchymal transition (EMT) in each group was observed under light microscope. The expression of E-cadherin, N-cadherin, Vimentin, NF-κB p65 and p-NF-κB p65 was detected by Western Blot. Results There was no statistically significant difference in miR-3651 level, activity of cells vitality, number of invasion cells, cells migration rate and cells morphology between mimic-NC group and control group (P>0.05), and there was no significant difference in the expression of E-cadherin, N-cadherin, Vimentin and p-NF-κB p65/NF-κB p65 proteins (P>0.05). Compared with the mimic-NC group, in the miR-3651 mimic group, miR-3651 level was significantly increased (P<0.05), cells vitality were significantly decreased at 2d, 3d, 4d after cell culture (P<0.05), number of invasion cells and cells migration rate were significantly decreased (P<0.05), expression of E-cadherin protein was significantly increased (P<0.05), expression of N-cadherin, Vimentin and p-NF-κB p65/NF-κB p65 proteins were significantly decreased (P<0.05), and EMT formation under microscope was decreased. Conclusions miR-3651 overexpression can inhibit the growth and invasion of CAL27 cells, and its action mechanism may be related to the inhibiting the phosphorylation of NF-κB signaling pathway.

Key words: microRNA-3651; , , Nuclear factor κB; , , Tongue cancer; , , Invasion

CLC Number:

R739.86

Lv Yanli , Ba Kai, Fang Zheng. microRNA-3651 overexpression inhibiting the growth and invasion of human tongue cancer CAL27 cells by mediating nuclear factor κB signaling pathway[J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 194-199.

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microRNA-3651 overexpression inhibiting the growth and invasion of human tongue cancer CAL27 cells by mediating nuclear factor κB signaling pathway

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