Macamide B促进缺氧缺血性脑损伤新生小鼠神经元的PGRN表达

doi:10.13418/j.issn.1001-165x.2023.3.10

Abstract

Abstract: Objective To investigate the effect of Macamide B on the expression of progranulin (PGRN) in neurons of neonatal mice with hypoxic-ischemic brain damage (HIBD) and HT22 mouse hippocampal neuron cell line after oxygen-glucose deprivation (OGD) and explore the mechanism of the neuroprotective effect of Macamide B after HIBD in neonatal mice. Methods The HIBD model of C57 mice was established after 7 days of birth and the mice were divided into Sham group, HIBD group and Macamide B group. HT22 cells were cultured, then the OGD model of HT22 cells was established, and the cells were divided into Control group, OGD group and Macamide B group. The expression of PGRN in mouse neurons or HT22 cells was detected by immunofluorescence staining and Western blot. Results In vivo experiments showed that compared with Sham group, the expression of PGRN in mice neurons of HIBD group was reduced. Compared with HIBD group, the expression of PGRN in neurons of Macamide B group was increased. In vitro, compared with Control group, the expression of PGRN in HT22 cells of OGD group was decreased. Compared with OGD group, the expression of PGRN in HT22 cells of Macamide B group rose significantly. Conclusions Macamide B may play a neuroprotective role in HIBD neonatal mice by promoting the expression of PGRN.

Key words: Hypoxic-ischemic brain damage; , , Macamide B; , , Neuroprotection; , , PGRN

CLC Number:

R722.1

Zhou Qian, Li Lixia, Yang Xiaoxia, Yang Junhua, Wen Jinkun, Li Pengfei, Luo Li, Li Guoying. Macamide B promoting the expression of PGRN in hypoxic-ischemic brain damage neonatal mice [J]. Chinese Journal of Clinical Anatomy, 2023, 41(3): 304-309.

References 15

[1]	Narayanamurthy R, Yang JL J, Yager JY, et al. Drug delivery platforms for neonatal brain injury[J]. J Control Release, 2021, 330(2): 765-787. DOI: 10.1016/j.jconrel.2020.12.056.
[2]	Zhu JJ, Yu BY, Huang XK, et al. Neferine protects against hypoxic-ischemic brain damage in neonatal rats by suppressing NLRP3-mediated inflammasome activation[J]. Oxid Med Cell Longev, 2021, 2021(5): 6654954. DOI: 10.1155/2021/6654954.
[3]	Kim BH, Jeziorek M, Kanal HD, et al. Moderately inducing autophagy reduces tertiary brain injury after perinatal hypoxia-ischemia[J]. Cells, 2021, 10(4): 898. DOI: 10.3390/cells10040898.
[4]	朱渝, 魏静, 吴鹏程, 等. 山莨菪碱缓解幼龄鼠缺氧缺血性脑组织形态和功能损伤[J]. 中国临床解剖学杂志, 2021, 39(5): 557-562, 568. DOI: 10.13418/j.issn.1001-165x.2021.05.011.
[5]	Zhou Y, Li P, Brantner A, et al. Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients[J]. Sci Rep, 2017, 7: 44660. DOI: 10.1038/srep44660.
[6]	Zhou Y, Wang H, Guo F, et al. Integrated proteomics and lipidomics investigation of the mechanism underlying the neuroprotective effect of N-benzylhexadecanamide[J]. Molecules, 2018, 23(11): 2929. DOI: 10.3390/molecules23112929.
[7]	Yang X, Wang M, Zhou Q, et al. Macamide B pretreatment attenuates neonatal hypoxic-ischemic brain damage of mice induced apoptosis and regulates autophagy via the PI3K/AKT signaling pathway[J]. Mol Neurobiol, 2022, 59(5): 2776-2798. DOI: 10.1007/s12035-022-02751-4.
[8]	Lan J, Hu Y, Wang X, et al. Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model[J]. Neuropharmacology, 2021, 196(2021): 108689. DOI: 10.1016/j.neuropharm.2021.108689.
[9]	张万平, 秦艳, 张海鹰, 等. 七氟醚对脑缺血再灌注损伤大鼠认知功能及海马S100β及PGRN表达的影响[J]. 现代生物医学进展, 2021, 21(17): 3222-3226. DOI: 10.13241/j.cnki.pmb.2021.17.005.
[10]	Wang L, Chen J, Hu Y, et al. Progranulin improves neural development via the PI3K/Akt/GSK-3β pathway in the cerebellum of a VPA-induced rat model of ASD[J]. Transl Psychiatry, 2022, 12(1): 114. DOI: 10.1038/s41398-022-01875-4.
[11]	Altmann C, Hardt S, Fischer C, et al. Progranulin overexpression in sensory neurons attenuates neuropathic pain in mice: role of autophagy[J]. Neurobiol Dis, 2016, 96: 294-311. DOI: 10.1016/j.nbd.2016.09.010.
[12]	Chen SX, Li KK, Pubu D, et al. Optimization of ultrasound-assisted extraction, HPLC and UHPLC-ESI-Q-TOF-MS/MS analysis of main macamides and macaenes from maca (cultivars of lepidium meyenii walp)[J]. Molecules, 2017, 22(12): 2196. DOI: 10.3390/molecules221 22196.
[14]	Doke M, Matsuwaki T, Yamanouchi K, et al. Lack of estrogen receptor α in astrocytes of progranulin-deficient mice[J]. J Reprod Dev, 2016, 62(6): 547-551. DOI: 10.1262/jrd.2016-067.
[15]	Wang Y, Wang X, Li Y, et al. Regulation of progranulin expression and location by sortilin in oxygen-glucose deprivation/reoxygenation injury[J]. Neurosci Lett,2020, 738: 135394. DOI: 10.1016/j.neulet. 2020. 135394.

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Macamide B promoting the expression of PGRN in hypoxic-ischemic brain damage neonatal mice

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