microRNA-125a靶向sortilin抑制巨噬细胞脂质蓄积与主动脉粥样硬化病变机制研究

doi:10.13418/j.issn.1001-165x.2023.4.12

Abstract

Abstract: Objective To investigate the effect and mechanism of miR-125a on the lipid metabolism of macrophages and the development of aortic atherosclerosis (AS) in LDLR?/? mice. Methods The lipid accumulation in macrophages was evaluated by Oil red O staining and colorimetry. The bioinformatics was used to predict the target gene of miR-125a. Double luciferase reporter gene analyzed the targeted binding between miR-125a and SORT1. The expressions of miR-125a and sortilin were detected in lipid-laden THP-1 macrophages and low-density lipoprotein receptor knockout (LDLR?/?) mice by qPCR、Western blot and immunohistochemistry. The blood lipid level was measured with automatic biochemical analyzer. The aortic AS plaque and lipid accumulation were showed by histological staining. Results Overexpression of miR-125a reduced lipid accumulation in THP-1 macrophages. Bioinformatics analysis and luciferase reporter gene showed that sortilin mRNA was a potential target of miR-125a. Overexpression of miR-125a suppressed sortilin expression and lipid accumulation in macrophages. miR-125a also ameliorated the blood lipid profile and reduced the plaque area of aortic AS in LDLR?/? mice. Conclusions miR-125a inhibits macrophage lipid accumulation and the progression of aortic AS by down-regulating sortilin expression.

Key words: miR-125a; , , sortilin; , , Macrophages; , , Lipid accumulation; , , Atherosclerosis

CLC Number:

Lv Yuncheng, Ming Xinyue, Liu Fang, Chen Shirui, Peng Tianhong, Ouyang Shuhui. MicroRNA-125a-targeted sortilin suppresses macrophage lipid accumulation and aortic atherogenesis[J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 440-446.

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MicroRNA-125a-targeted sortilin suppresses macrophage lipid accumulation and aortic atherogenesis

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