腹内侧前额叶皮质锥体神经元参与选择性坐骨神经损伤小鼠疼痛和焦虑样行为调控的研究

doi:10.13418/j.issn.1001-165x.2023.5.10

Abstract

Abstract: Objective To explore the role of pyramidal neurons of ventromedial prefrontal cortex (vmPFC) in pain and anxiety-like behavior of spared nerve injury (SNI) mice. Methods Spared nerve injury (SNI) was used to mimic neuropathic pain (NP) animal model, while a same incision without nerve injury was performed to establish the sham control group. Kainic aicd, normal saline, AAV2/9 virus were intracranially injected into contralateral vmPFC of SNI mice. Von frey filaments were used to detect the paw withdraw threshold (PWT) of the ipsilateral planta of SNI mice. The elevated plus maze (EPM) was used to assess anxiety behaviors of SNI mice. Immunofluorescence staining was used to observe the expression of c-fos, the neuronal loss and the astrocytic activation in the contralateral vmPFC. RNA-scope and immunofluorescence staining were used to identify the neuronal type of c-fos positive neuron of vmPFC. Results Compared with sham mice, ipsilateral PWT, entries in open arms and cumulative duration in open arms of SNI mice were significantly decreased at 7d and 14d after SNI (P<0.05). Compared with sham mice, c-fos positive neurons were significantly increased in the contralateral of vmPFC of SNI mice (P<0.05). In addition, the c-fos positive neurons were almost Vglut1 positive neurons. Compared with the SNI mice which intracranially injected into contraleral vmPFC with normal saline, ipsilateral PWT, entries in open arms and cumulative duration in open arms of the SNI mice which intracranially injected into contraleral vmPFC with kainic acid were increased (P<0.05). Meanwhile, the SNI mice which intracranially injected into contraleral vmPFC with kainic acid exhibited obvious neuronal loss and astrocytic activation in the contralateral vmPFC. In addition, using chemogenetics strategy to inhibit the pyramidal neurons in contralateral vmPFC of SNI mice, the c-fos positive neurons were significantly deceased, the ipsilateral PWT, entries in open arms and cumulative duration in open arms were significantly increased (P<0.05). Conclusions Pyramidal neurons in the contralateral vmPFC may be involved in the development of pain and anxiety-like behaviors of SNI mice.

Key words: Ventromedial prefrontal cortex; , , Chemogenetics; , , Pyramidal neuron; , , Pain; , , Anxiety

CLC Number:

R338

Lin Peimin, Liu Yan, Liu Ting, Yin Haoling, Zhang Ying . The involvements of pyramidal neurons in ventromedial prefrontal cortex in the regulation of pain and anxiety-like behavior of spared nerve injury mice[J]. Chinese Journal of Clinical Anatomy, 2023, 41(5): 557-564.

References 19

[1]	杜涛, 袁文茜, 曹伯旭, 等. 慢性神经病理性疼痛[J]. 中国疼痛医学杂志, 2021, 27(7): 481-485. DIO: 10.3969/j.issn.1006-9852.2021.07.001.
[2]	Li KL, Chen YM, Wang XQ, et al. Bibliometric analysis of studies on neuropathic pain associated with depression or anxiety published from 2000 to 2020[J]. Front Hum Neurosci, 2021, 15: 729587. DOI: 10.3389/fnhum.2021.729587.
[3]	Kim D, Mirmina J, Narine S, et al. Altered physical pain processing in different psychiatric conditions[J]. Neurosci Biobehav Rev, 2022, 133: 104510. DOI: 10.1016/j.neubiorev.2021.12.033.
[4]	Jalbrzikowski M, Larsen B, Hallquist M, et al. Development of white matter microstructure and intrinsic functional connectivity between the amygdala and ventromedial prefrontal cortex: associations with anxiety and depression[J]. Biol Psychiatry, 2017, 82(7): 511-521. DOI: 10.1016/j.biop syc h.2017.01.008.
[5]	Baliki MN, Petre B, Torbey S, et al. Corticostriatal functional connectivity predicts transition to chronic back pain[J]. Nat Neurosci, 2012, 15(8): 1117-1119. DOI: 10.1038/nn.3153.
[6]	Metz AE, Yau HJ, Centeno MV, et al. Morphological and functional reorganization of rat medial prefrontal cortex in neuropathic pain[J]. Proc Natl Acad Sci U S A, 2009, 106(7): 2423-2428. DOI: 10.1073/pnas.0809897106.
[7]	Nawreen N, Baccei ML, Herman JP. Single prolonged stress reduces intrinsic excitability and excitatory synaptic drive onto pyramidal neurons in the infralimbic prefrontal cortex of adult male rats[J]. Front Cell Neurosci, 2021, 15: 705660. DOI: 10.3389/fncel.2021.705660.
[8]	Liang HY, Chen ZJ, Xiao H, et al. nNOS-expressing neurons in the vmPFC transform pPVT-derived chronic pain signals into anxiety behaviors[J]. Nat Commun, 2020, 11(1): 2501. DOI: 10.1038/s41467-020-16198-5.
[9]	Decosterd I, Woolf CJ. Spared nerve injury: an animal model of persistent peripheral neuropathic pain[J]. Pain, 2000, 87(2): 149-158. DOI: 10.1016/S0304-3959(00)00276-1.
[10]	Pak DJ, Yong RJ, Kaye AD, et al. Chronification of pain: mechanisms, current understanding, and clinical implications[J]. Curr Pain Headache Rep, 2018, 22(2): 9. DOI: 10.1007/s11916-018-0666-8.
[11]	Huang J, Gadotti V, Chen L, et al. A neuronal circuit for activating descending modulation of neuropathic pain[J]. Nat Neurosci, 2019, 22(10): 1659-1668. DOI: 10.1038/s41593-019-0481-5.
[12]	Bushnell M, Ceko M, Low L. Cognitive and emotional control of pain and its disruption in chronic pain[J]. Nat Rev Neurosci, 2013, 14(7): 502-511. DOI: 10.1038/nrn3516.
[13]	Hiser J, Koenigs M. The multifaceted role of the ventromedial prefrontal cortex in emotion, decision making, social cognition, and psychopathology[J]. Biol Psychiatry, 2018, 83(8): 638-647. DOI: 10.1016/j.biopsych.2017.10.030.
[14]	Yin J, Liang S, Li F, et al. dmPFC-vlPAG projection neurons contribute to pain threshold maintenance and antianxiety behaviors[J]. J Clin Invest, 2020, 130(12): 6555-6570. DOI: 10.1172/JCI127607.
[15]	许祥影, 沈晶晶, 朱贺, 等. 硫辛酸对炎性痛小鼠脊髓背角c-Fos和Iba-1的影响[J]. 中国疼痛医学杂志, 2019, 25(9): 655-659, 665. DOI: 10.3969/j.issn.1006-9852.2019.09.004.
[16]	宿世玉, 田恩琪, 党秀静, 等. 触液核在大鼠慢性瘙痒维持中的作用[J]. 中华麻醉学杂志, 2013, 33(7): 807-809. DOI: 10.3760/cma.j.issn.0254-1416.2013.07.007.
[17]	Bi LL, Wang J, Luo ZY, et al. Enhanced excitability in the infralimbic cortex produces anxiety-like behaviors[J]. Neuropharmacology, 2013, 72: 148-156. DOI: 10.1016/j.neuropharm.2013.04.048.
[18]	Felix-Ortiz AC, Beyeler A, Seo C, et al. BLA to vHPC inputs modulate anxiety-related behaviors[J]. Neuron, 2013, 79(4): 658-664. DOI: 10.1016/j.neuron.2013.06.016.
[19]	Visser E, Matos MR, Mitrić MM, et al. Extinction of cocaine memory depends on a feed-forward inhibition circuit within the medial prefrontal cortex[J]. Biol Psychiatry, 2022, 91(12): 1029-1038. DOI: 10.1016/j.biopsych.2021.08.008.

The involvements of pyramidal neurons in ventromedial prefrontal cortex in the regulation of pain and anxiety-like behavior of spared nerve injury mice

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 19

Related Articles 15

Metrics

Comments

Recommended 0

[1]	Jiang Jianhong, Duan Renpeng, Li Xiaofeng. Application of three-dimensional visualization technology in the anatomical variations of hilar bile ducts in Chinese population [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 1-4.
[2]	Guo Hongzhi, Wang Yu, Zhang Jiaqi, Liang Haibin, Ma Ziwei, Feng Wei, Wu You, Si Ziyi. Evaluation of placental vascular structure in preeclampsia by vascular casting combined with CT three-dimensional model [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 5-10.
[3]	Li Jiawei, Zhang Jing, Li Canran, Lan Wenjie, Ji Qingyu, Guo Zhiyong, Zhang Yunfeng, Liu Qi, Chen Qingwei, Li Xiaohe. X-ray measurement of proximal femur anatomical parameters in Mongolian population [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 11-16.
[4]	. Digital measurement and clinical significance of occipitocervical Angle and posterior occipitocervical Angle in children and adolescents [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 17-20.
[5]	Li Wen, Yang Siyi, Huang Lei, Qing Jiwen, Jiang Songtao, Zhang Lei. Morphological characteristics and clinical significance of Lisfranc ligament based on MRI [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 21-25.
[6]	Li Fanfan, Xu Yang, Wang Xiaoxu. Therapeutic effect of shikonin on the treatment of granuloma lobular mastitis in model rats by regulating Nrf2/HO-1 signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 26-32.
[7]	Wang Xinghang, Ding Jiayuan, Li Fang, Bao Cuifen, Yan Lijing. Ginsenoside Rg1 reduces the inflammatory response of microglia after oxygen glucose deprivation/resupply by inhibiting the NLRP3 inflammasome pathway [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 33-41.
[8]	Cheng Yingying, Wu Jianjun, Cai Haiyan , Jiao Xunwen, Ma Jiangbo, Ding Yinxiu. Activation and functional changes of astrocytes cultured in vitro under inflammatory stimulation [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 42-46.
[9]	Qi Zhi, Yang Li, Jia Qiuye, Chen Haolun, Duan Zhaoda, Wu Chunyun. Edaravone regulates Sirt3 expression in lipopolysaccharide-activated microglia via MAPKs signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 47-53.
[10]	Zhang Jingjing, Wang Hongxin. Protective effect of Baicalin on rats with pulmonary hypertension through PDGF/P38 MAPK signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 54-58.
[11]	Yue Qin, Chen Rongli, Xiong Jingxi, Wang Tingyi, Cai Zhiheng, Yi Qiushi, Zeng Xinyi. Effect of Bletilla striata polysaccharides on the choke-area of cross-boundary flap in rats [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 59-64.
[12]	Li Xiaoyu , Zhang Lei , Fu Lei , Li Dongbo , Wang Guoyou. Finite element study on the treatment of Sanders type II calcaneal fracture with three-step reduction method [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 65-70.
[13]	Wu Yanfei, Ma Jianxiong, Lu Bin, Wang Ying, Bai Haohao, Jin Hongzhen, Ma Xinlong. Study on the correlation between the reconstruction of grayscale values using CT post-processing technology and the compressive strength of the endplate [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 71-76.
[14]	Zhuang Wanqiang, Tang Yi, Luo Yonggang, Zhang Hui. An early and mid-term retrospective study of the effects of arthroscopy combined with high tibial osteotomy on patellar position and patellofemoral joint function [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 77-82.
[15]	Luo Ying, Dou Weiyu, Wu Xiaohang, Chen Jingkun, Peng Changgui, Pan Jianying . Risk factors of postoperative complications of calcaneal fractures treated with hippocampal plate internal fixation through minimally invasive method [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 83-88.