正畸牙移动中脂联素调控PI3K/AKt信号通路对牙周组织改建的影响

doi:10.13418/j.issn.1001-165x.2023.5.14

Abstract

Abstract: Objective To investigate the effect of adiponectin (APN) regulating phosphatidylinositol 3 kinase (PI3K) / protein serine threonine kinase (AKt) signal pathway on periodontal tissue remodeling during orthodontic tooth movement. Methods Sixty rats were randomly divided into a blank group (untreated), a PBS group (injected with PBS), an APN group (injected with APN) and an APN + insulin growth factor-1 (IGF-1) (PI3K / Akt pathway activator) group, 15 mice in each group. The movement distance of the first molar and the number of osteoclasts in the three groups were observed at 1d, 7 d and 14 d, and the morphological changes of periodontal tissue in the three groups were observed at 14 d. The expressions of PI3K and AKt mRNA and PI3K, p-Pi3K, AKt and p-AKt protein in periodontal tissue were compared. Results At 7d and 14d, the movement distance of the first molar in APN group was significantly lower than that in blank group (P<0.05). The amount of new bone formation and the degree of new bone mineralization in APN group were higher than those in blank group and PBS group. At 7d and 14d, the number of osteoclasts in APN group was significantly lower than that in blank group (P<0.05). The expressions of PI3K, AKt mRNA and p-Pi3K, p-AKt protein in periodontal tissue in APN group were significantly lower than those in blank control group (P<0.05). The expressions of PI3K, AKt mRNA and p-Pi3K, p-AKt protein in APN + IGF-1 group were significantly higher than those in APN group (P<0.05). Conclusions APN can reduce the number of osteoclasts on the pressure side and the speed of tooth movement in the rat model of orthodontic tooth movement, and plays an important role in delaying the remodeling of periodontal tissue during orthodontic tooth movement. Its mechanism may be realized by inhibiting PI3K / AKt signal pathway

Key words: Orthodontic tooth movement; , , Adiponectin; , , Phosphatidylinositol 3 kinase; , , Protein serine threonine kinase; , , Periodontal tissue remodeling

CLC Number:

R783.5

Xu Peiqiong, Zheng Zhiguo, Wu Jun. Effect of adiponectin regulating PI3K / AKt signaling pathway on periodontal tissue remodeling during orthodontic tooth movement[J]. Chinese Journal of Clinical Anatomy, 2023, 41(5): 583-587.

References 21

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Effect of adiponectin regulating PI3K / AKt signaling pathway on periodontal tissue remodeling during orthodontic tooth movement

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