Abstract: Objective　 To observe the vascular leak prevention and cranial bone fixation effects of polyurethane foam adhesive during the preservation and perfusion of fresh gross specimens after craniotomy. Method    Reset the skull cap, suture the scalp continuously, fix it with a zip tie along the mandible through the ear to the top of the skull, and fill it with intracranial polyurethane foam adhesive; Bilateral catheterization of the femoral artery was performed, and gravity and micro pumps were used for perfusion. The leakage rate of the skull section was measured to evaluate the effectiveness of polyurethane foam adhesive in preventing vascular leakage and fixing the skull cap on fresh gross specimens after removal.   Result   The proportion of skull section leakage to the total perfusion fluid is relatively low, and the effect of vascular leak prevention is significant; After drying, the foam adhesive tightly adheres to the cranial bone and evenly covers all holes and fissures in the skull base.   Conclusion    Polyurethane foam adhesive has an ideal effect on the preservation, perfusion, leak prevention, and fixation of fresh gross specimens after craniotomy, which is beneficial for the recovery of the donor's residual appearance and contributes to the construction and development of the human brain tissue bank.

Key words: Polyurethane foam adhesive, 　Vascular leak prevention, 　Human brain tissue library