Abstract: Objective    To investigate the effect of Zhuang Medicine Fuzheng Compound on the exhaustion of CD8+ T cells infiltrated by lung cancer and the efficacy of PD-L1 inhibitors, and to clarify the possible mechanism.    Methods    MTT and plate cloning were used to detect the effect of Zhuang Medicine Fuzheng Compound on the proliferation of LLC cells of mouse lung cancer. Flow cytometry was used to detect the proportion of CD8+T and the expression level of PD-L1, and Western blot was used to detect the expression level of protein to preliminarily explore the relevant mechanism. Finally, the synergistic effect of Zhuang Medicine Fuzheng Compound and PD-L1 inhibitor was verified by animal experiments.    Results Zhuang Medicine Fuzheng Compound inhibited the proliferation of LLC cells in mice. In vivo experiments in mice, Zhuang Medicine Fuzheng Compound and PD-L1 inhibitor could play a synergistic anti-tumor effect. Zhuang Medicine Fuzheng Compound delayed the depletion of CD8+ T cells and inhibited the expression of PD-L1. Zhuang Medicine Fuzheng Compound could reduce the activity of PI3K/AKT pathway.    Conclusions    Zhuang Medicine Fuzheng Compound can delay CD8+ T cell depletion and inhibit PD-L1 expression, and enhance the efficacy of PD-L1 inhibitors in the treatment of lung cancer, which may be related to the reduction of PI3K/AKT signaling pathway.

Key words: Zhuang Medicine Fuzheng Compound; ,  Lung cancer; ,  TIL cell depletion; ,  , PI3K/AKT signaling pathway; ,  , Proliferation