Abstract:

Objective　To explore the protective effect of modified recombinant hunan aFGF(Mrh-aFGF) on the neurons in substantia nigra of Parkinson disease rats.   Methods　The Parkinson's disease( PD) rats were induced by injecting 6-OHDA into the left substantia nigra compacta (SNC) and ventral tegmental area(VTA), Then, the PD rats were treated with Mrh-aFGF or saline by lateral ventricle injection. The behavioral changes of the treated rats were detected after apomorphine injection. The morphologic features of the neurons in the substantia nigra were observed by Nissl's staining and electronic microscopy.   Results　Rotation behavior was not found in the intacted rats, otherwise, the average rotation rate speeded by degrees in PD and NS groups, however, rats treated with Mrh-aFGF showed slower rotation rate (P<0.05). In PD and NS groups, the neurons in left（the injured side）SNC of PD rats decreased significantly ( P<0.05) compared to that of right side. In Mrh-aFGF group, the structure of the left（the injured side）SNC was significantly improved and the number of neurons was increased, compared with that of PD and NS groups (P<0.05). The nigral neurons of PD rats appeared karyopyknosis, the swollen mitochondria, and the crista lost, as well, endoplasmic reticulum was found to be dilatated and degranulated obviouly, moreover, pre-synaptic and post-synaptic membranes were not clear and the synaptic space disappeared. In Mrh-aFGF group, the ultrastructure of nigral neurons, such as nuclei, mitochondria, synaptic structure, kept well compared to that of PD group.   Conclusions　Mrh-aFGF could modify the rotation behavior, protect nigral neurons from the loss and keep the normal ultrastructures of nigral neurons of Parkinson disease rats.

Key words: Parkinson disease!Modified recombinant human acid fibroblast growth factor(Mrh-aFGF)! Substantia , nigra !Ultrastructure!Rat