CK19和TGF-α在大鼠肝纤维化部分肝切除后残肝中的表达

Abstract

Abstract:

Objective　To detect the expressions of CK19 and TGF-α in reserved liver after partial hepatectomy (PH) of rat with hepatic fibrosis at the different time phase, for evaluating the bile canaliculi regeneration in fibrotic liver tissue. Methods　Male SD rats were divided into two groups: the control group (35 rats) and the experimental group (35 rats). The rats of experimental group were injected i.p. with CCl4 to establish the hepatic fibrosis model, and then animals from two groups was endured PH operation. The liver tissues were obtained from each group at 0, 1, 3, 5 and 7d after PH, followed by immunohistochemical analysis. Results (1) The expression of CK19 increased gradually after PH in both groups, however, in experimental group, the expression intensity was significantly higher than that of in control group at the same time point (P<0.05) except for that at 7d. As well, the expression of TGF-α increased gradually after PH in both groups, however, The expression was significant higher in the experimental group at 0 d, 1 d, 3 d and lower at 5 d, 7 d compared to that in the control group.　Conclusions　For rat model of hepatic fibrosis, the increased expression of CK19 can be detected in reserved liver after partial hepatectomy, which indicates that the proliferation and differentiation of HOCs can be promoted by hepatic fibrosis and PH treatment. In contrast, TGF-α is uneffective for promoting proliferation of HOCs and bile canaliculi regeneration.

Key words: Rat, Hepatic fibrosis, Bile canaliculi, CK19, TGF-α

CLC Number:

R575

YUE Xue-Qiang, SHI Xiang-Kong, LI Na-Na, ZANG Wei-Dong, LIU Heng-Xing. Expressions of CK19 and TGF-α in reserved liver after partial hepatectomy of rat with hepatic fibrosis[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(2): 208-212.

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Expressions of CK19 and TGF-α in reserved liver after partial hepatectomy of rat with hepatic fibrosis

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